| Literature DB >> 26883252 |
Fei Cui1, Duoguang Wu2, Xiaotian He2, Wenjian Wang2, Jingle Xi1, Minghui Wang3.
Abstract
The biology of esophageal squamous cell carcinoma (ESCC) remains poorly understood. Long noncoding RNAs (lncRNAs) are found to be dysregulated in a variety of cancers, including ESCC. SPRY4-IT1 has been recently revealed as oncogenic regulator or tumor suppressors in different cancers; however, whether SPRY4-IT1 is involved in ESCC remains poorly understood. To investigate the role of SPRY4-IT1 in ESCC, we evaluated the SPRY4-IT1 expression levels in a series of ESCC patients and a panel of ESCC cell line using qRT-PCR. CCK8 and colony formation assay were performed to assess the effect of SPRY4-IT1siRNA on cell proliferation, migration, and invasion of ESCC cell lines. SPRY4-IT1 expression was upregulated in ESCC tissues and the higher expression of SPRY4-IT1 was significantly correlated with tumor grade, depth of invasion, and lymph node metastasis. Moreover, silencing of SPRY4-IT1 expression inhibited ESCC cell proliferation, colony formation, migration, and invasion. Therefore, our study indicates that SPRY4-IT1 promotes proliferation and migration of ESCC cells and is a potential oncogene of ESCC.Entities:
Keywords: EMT; Esophageal squamous cell carcinoma; Long noncoding RNA; SPRY4-IT1
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Year: 2016 PMID： 26883252 DOI： 10.1007/s13277-016-4962-9
Source DB: PubMed Journal: Tumour Biol ISSN： 1010-4283