Immunogenic and protective properties of rabies virus glycoprotein expressed by baculovirus vectors.

The gene encoding the glycoprotein of rabies virus (G protein, CVS strain) has been cloned and inserted into the baculovirus transfer vector pAcYM1 derived from the nuclear polyhedrosis virus of Autographa californica (AcNPV). The gene was placed under the control of the AcNPV polyhedrin promoter and expressed to high levels by the derived recombinant virus using a Spodoptera frugiperda cell line. It has been established that the antigenic characteristics of the protein were conserved by comparison with those of the native glycoprotein of rabies virions. The immunogenicity of the expressed product was also demonstrated. Intraperitoneal or intramuscular injection of G antigen conferred protection to mice and was associated with the induction of high titers of neutralizing antibodies. The availability of large quantities of antigenically and immunogenically reactive rabies G protein may make feasible crystallographic studies and the safe preparation of a low cost subunit vaccine for the disease.