Megastigmane Glycosides from the Leaves of Tripterygium wilfordii.

Two new megastigmane glycosides, named wilfordonisides A and B (1 and 2), and four known compounds (3-6) were isolated from the leaves of Tripterygium wilfordii, and one new aglycon, named wilfordoninol A (2a), was acquired by enzymatic hydrolysis of 2. The absolute stereostructures of the compounds were determined by Mosher's method and by CD. At a concentration of 10 μM, compounds 1, 3, and 5 inhibited STAT1 translocation by 38.1 ± 0.9%, 55.8 ± 0.8%, and 53.9 ± 1.0%, respectively.