Raisa Syed1, Noreene M Shibata1, Kusum K Kharbanda2, Ruijun J Su3, Kristin Olson3, Amy Yokoyama4, John C Rutledge4, Kenneth J Chmiel1, Kyoungmi Kim5, Charles H Halsted1, Valentina Medici1. 1. 1 Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of California , Davis, Sacramento, California. 2. 2 Research Service, Veterans Affairs Nebraska-Western Iowa Health Care System , Omaha, Nebraska. 3. 3 Department of Pathology and Laboratory Medicine, University of California , Davis, Sacramento, California. 4. 4 Division of Cardiovascular Medicine, Department of Internal Medicine, University of California , Davis, Davis, California. 5. 5 Division of Biostatistics, Department of Public Health Sciences, University of California , Davis, Davis, California.
Abstract
BACKGROUND: Previous studies indicated that nonpurified and purified commercially available control murine diets have different metabolic effects with potential consequences on hepatic methionine metabolism and liver histology. METHODS: We compared the metabolic and histological effects of commercial nonpurified (13% calories from fat; 57% calories from carbohydrates with 38 grams/kg of sucrose) and purified control diets (12% calories from fat; 69% calories from carbohydrates with ∼500 grams/kg of sucrose) with or without choline supplementation administered to C3H mice with normal lipid and methionine metabolism. Diets were started 2 weeks before mating, continued through pregnancy and lactation, and continued in offspring until 24 weeks of age when we collected plasma and liver tissue to study methionine and lipid metabolism. RESULTS: Compared to mice fed nonpurified diets, the liver/body weight ratio was significantly higher in mice fed either purified diet, which was associated with hepatic steatosis and inflammation. Plasma alanine aminotransferase levels were higher in mice receiving the purified diets. The hepatic S-adenosylmethionine (SAM)/S-adenosylhomocysteine (SAH) ratio was higher in female mice fed purified compared to nonpurified diet (4.6 ± 2 vs. 2.8 ± 1.9; P < 0.05). Choline supplementation was associated with improvement of some parameters of lipid and methionine metabolism in mice fed purified diets. CONCLUSIONS: Standard nonpurified and purified diets have significantly different effects on development of steatosis in control mice. These findings can help in development of animal models of fatty liver and in choosing appropriate laboratory control diets for control animals.
BACKGROUND: Previous studies indicated that nonpurified and purified commercially available control murine diets have different metabolic effects with potential consequences on hepatic methionine metabolism and liver histology. METHODS: We compared the metabolic and histological effects of commercial nonpurified (13% calories from fat; 57% calories from carbohydrates with 38 grams/kg of sucrose) and purified control diets (12% calories from fat; 69% calories from carbohydrates with ∼500 grams/kg of sucrose) with or without choline supplementation administered to C3H mice with normal lipid and methionine metabolism. Diets were started 2 weeks before mating, continued through pregnancy and lactation, and continued in offspring until 24 weeks of age when we collected plasma and liver tissue to study methionine and lipid metabolism. RESULTS: Compared to mice fed nonpurified diets, the liver/body weight ratio was significantly higher in mice fed either purified diet, which was associated with hepatic steatosis and inflammation. Plasma alanine aminotransferase levels were higher in mice receiving the purified diets. The hepatic S-adenosylmethionine (SAM)/S-adenosylhomocysteine (SAH) ratio was higher in female mice fed purified compared to nonpurified diet (4.6 ± 2 vs. 2.8 ± 1.9; P < 0.05). Choline supplementation was associated with improvement of some parameters of lipid and methionine metabolism in mice fed purified diets. CONCLUSIONS: Standard nonpurified and purified diets have significantly different effects on development of steatosis in control mice. These findings can help in development of animal models of fatty liver and in choosing appropriate laboratory control diets for control animals.
Authors: Natalie C Chen; Fan Yang; Louis M Capecci; Ziyu Gu; Andrew I Schafer; William Durante; Xiao-Feng Yang; Hong Wang Journal: FASEB J Date: 2010-03-19 Impact factor: 5.191
Authors: Valentina Medici; Noreene M Shibata; Kusum K Kharbanda; Janine M LaSalle; Rima Woods; Sarah Liu; Jesse A Engelberg; Sridevi Devaraj; Natalie J Török; Joy X Jiang; Peter J Havel; Bo Lönnerdal; Kyoungmi Kim; Charles H Halsted Journal: Hepatology Date: 2013-01-10 Impact factor: 17.425
Authors: Anh Le; Noreene M Shibata; Samuel W French; Kyoungmi Kim; Kusum K Kharbanda; Mohammad S Islam; Janine M LaSalle; Charles H Halsted; Carl L Keen; Valentina Medici Journal: Int J Mol Sci Date: 2014-05-07 Impact factor: 5.923