Literature DB >> 2687880

Vectorial targeting of apical and basolateral plasma membrane proteins in a human adenocarcinoma epithelial cell line.

A Le Bivic1, F X Real, E Rodriguez-Boulan.   

Abstract

We studied the surface delivery pathways followed by newly synthesized plasma membrane proteins in intestinal cells. To this end, we developed an assay and characterized an epithelial cell line (SK-CO-15) derived from human colon adenocarcinoma. Polarized confluent monolayers (2000 omega.cm2), grown on polycarbonate filter chambers, were pulsed with radioactive methionine/cysteine and, at different times of chase, the protein fraction reaching the apical or basolateral surface was recovered by domain-selective biotinylation, immunoprecipitation, and immobilized streptavidin precipitation. Both an apical and a basolateral marker were found to be delivered vectorially to the respective surface, with a sorting efficiency of 50:1 for the basolateral marker and 14:1 for the apical marker.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2687880      PMCID： PMC298485          DOI： 10.1073/pnas.86.23.9313

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  38 in total

1.  Transepithelial transport in cell culture.

Authors:  D S Misfeldt; S T Hamamoto; D R Pitelka
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

2.  Biogenesis of intestinal plasma membrane: posttranslational route and cleavage of sucrase-isomaltase.

Authors:  H P Hauri; A Quaroni; K J Isselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors:  J P Chamberlain
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

5.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

6.  Phase separation of integral membrane proteins in Triton X-114 solution.

Authors:  C Bordier
Journal:  J Biol Chem       Date:  1981-02-25       Impact factor: 5.157

7.  Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.

Authors:  A L Tarentino; F Maley
Journal:  J Biol Chem       Date:  1974-02-10       Impact factor: 5.157

8.  Synthesis of membrane glycoproteins in rat small-intestinal villus cells. Redistribution of L-[1,5,6-3H]fucose-labelled membrane glycoproteins among Golgi, lateral basal and microvillus membranes in vivo.

Authors:  A Quaroni; K Kirsch; M M Weiser
Journal:  Biochem J       Date:  1979-07-15       Impact factor: 3.857

9.  Localization by immunofluorescence and histochemical labeling of aminopeptidase N in relation to its biosynthesis in rabbit and pig enterocytes.

Authors:  H Feracci; A Bernadac; J P Gorvel; S Maroux
Journal:  Gastroenterology       Date:  1982-02       Impact factor: 22.682

10.  A technique for ultracryotomy of cell suspensions and tissues.

Authors:  K T Tokuyasu
Journal:  J Cell Biol       Date:  1973-05       Impact factor: 10.539
View more
  86 in total

1.  Rotavirus spike protein VP4 is present at the plasma membrane and is associated with microtubules in infected cells.

Authors:  M Nejmeddine; G Trugnan; C Sapin; E Kohli; L Svensson; S Lopez; J Cohen
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

2.  Characterization of a di-leucine-based signal in the cytoplasmic tail of the nucleotide-pyrophosphatase NPP1 that mediates basolateral targeting but not endocytosis.

Authors:  V Bello; J W Goding; V Greengrass; A Sali; V Dubljevic; C Lenoir; G Trugnan; M Maurice
Journal:  Mol Biol Cell       Date:  2001-10       Impact factor: 4.138

3.  The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.

Authors:  Gloria Salazar; Rachal Love; Erica Werner; Michele M Doucette; Su Cheng; Allan Levey; Victor Faundez
Journal:  Mol Biol Cell       Date:  2003-12-02       Impact factor: 4.138

4.  Expression of receptors for enterotoxigenic Escherichia coli during enterocytic differentiation of human polarized intestinal epithelial cells in culture.

Authors:  S Kernéis; G Chauvière; A Darfeuille-Michaud; D Aubel; M H Coconnier; B Joly; A L Servin
Journal:  Infect Immun       Date:  1992-07       Impact factor: 3.441

5.  Hypoxia enhances stimulus-dependent induction of E-selectin on aortic endothelial cells.

Authors:  G Zünd; D P Nelson; E J Neufeld; A L Dzus; J Bischoff; J E Mayer; S P Colgan
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-09       Impact factor: 11.205

Review 6.  Plasma membrane protein polarity and trafficking in RPE cells: past, present and future.

Authors:  Guillermo L Lehmann; Ignacio Benedicto; Nancy J Philp; Enrique Rodriguez-Boulan
Journal:  Exp Eye Res       Date:  2014-09       Impact factor: 3.467

7.  beta-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform.

Authors:  Elöd Z Szabó; Masayuki Numata; Viktoria Lukashova; Pietro Iannuzzi; John Orlowski
Journal:  Proc Natl Acad Sci U S A       Date:  2005-02-07       Impact factor: 11.205

8.  Exocyst Sec10 is involved in basolateral protein translation and translocation in the endoplasmic reticulum.

Authors:  Soo Young Choi; Ben Fogelgren; Xiaofeng Zuo; Liwei Huang; Sarah McKenna; Vishwanath R Lingappa; Joshua H Lipschutz
Journal:  Nephron Exp Nephrol       Date:  2012-10-04

9.  Hepatocyte growth factor/scatter factor effects on epithelia. Regulation of intercellular junctions in transformed and nontransformed cell lines, basolateral polarization of c-met receptor in transformed and natural intestinal epithelia, and induction of rapid wound repair in a transformed model epithelium.

Authors:  A Nusrat; C A Parkos; A E Bacarra; P J Godowski; C Delp-Archer; E M Rosen; J L Madara
Journal:  J Clin Invest       Date:  1994-05       Impact factor: 14.808

10.  Actin is a surface component of calf pulmonary artery endothelial cells in culture.

Authors:  J Moroianu; J W Fett; J F Riordan; B L Vallee
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.