Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Direct observation of a 91 bp LacI-mediated, negatively supercoiled DNA loop by atomic force microscope.

Literature DB >> 26878689

Direct observation of a 91 bp LacI-mediated, negatively supercoiled DNA loop by atomic force microscope.

Geraldine Fulcrand^1,2, Prem Chapagain^1,3, David Dunlap⁴, Fenfei Leng^1,2.

Abstract

Escherichia coli lactose repressor (LacI), a tetrameric protein, is a paradigmatic transcriptional factor that controls the expression of lacZYA in the lac operon. It specifically binds to the O1, O2, and O3 operators of the lac promoter, forms DNA loops, and regulates transcription of the lac operon. In this article, utilizing combined techniques of DNA-nicking assay and AFM imaging, we directly observed a 91 bp LacI-mediated, negatively supercoiled DNA loop mimicking the DNA loop between the O1 and O3 operators in the lac promoter.

Entities: Chemical Gene Species

Keywords: DNA loop; atomic force microscope; the lac operon; the lac promoter; the lac repressor

Mesh：

Substances：

Year: 2016 PMID： 26878689 PMCID： PMC4783203 DOI： 10.1002/1873-3468.12094

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

22 in total

Review 1. Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.

Authors: Fenfei Leng
Journal: Biophys Rev Date: 2016-06-10

Review 2. Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.

Authors: Fenfei Leng
Journal: Biophys Rev Date: 2016-11-14

3. Requirements for DNA-Bridging Proteins to Act as Topological Barriers of the Bacterial Genome.

Authors: Marc Joyeux; Ivan Junier
Journal: Biophys J Date: 2020-08-12 Impact factor: 4.033

3 in total

Direct observation of a 91 bp LacI-mediated, negatively supercoiled DNA loop by atomic force microscope.

1. Structural basis for cooperative DNA binding by CAP and lac repressor.

2. Dividing a supercoiled DNA molecule into two independent topological domains.

3. Repression of lac promoter as a function of distance, phase and quality of an auxiliary lac operator.

4. DNA supercoiling promotes formation of a bent repression loop in lac DNA.

5. The three operators of the lac operon cooperate in repression.

6. DNA linking number change induced by sequence-specific DNA-binding proteins.

7. Bacterial promoter repression by DNA looping without protein-protein binding competition.

8. AFM studies of lambda repressor oligomers securing DNA loops.

9. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer.

10. Effects of nucleoid proteins on DNA repression loop formation in Escherichia coli.

Review 1. Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.

Review 2. Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.

3. Requirements for DNA-Bridging Proteins to Act as Topological Barriers of the Bacterial Genome.