R Cooper1, L Jenkins1. 1. At Centre for Biomedical Sciences, Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB.
Abstract
OBJECTIVE: To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing in vitro. METHOD: Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm. RESULTS: Pseudomonas aeruginosa biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples. CONCLUSION: Biofilms of two different test bacteria bound to dressings in vitro with the DACC-coating on the dressings enhancing the binding of MRSA biofilm. DECLARATION OF INTEREST: This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.
OBJECTIVE: To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing in vitro. METHOD: Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm. RESULTS:Pseudomonas aeruginosa biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples. CONCLUSION: Biofilms of two different test bacteria bound to dressings in vitro with the DACC-coating on the dressings enhancing the binding of MRSA biofilm. DECLARATION OF INTEREST: This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.
Authors: Joshua P Totty; Louise H Hitchman; Paris L Cai; Amy E Harwood; Tom Wallace; Dan Carradice; George E Smith; Ian C Chetter Journal: Int Wound J Date: 2019-03-14 Impact factor: 3.315