Meat Quality of Loin and Top Round Muscles from the Hanwoo and Holstein Veal Calves.

This study was conducted to compare the meat qualities of loin (m. longissimus dorsi) and top round (m. semimembranosus) from Hanwoo and Holstein veal. Ten Hanwoo and Holstein calves were randomly selected from a local cattle farm and raised. They were slaughtered when they were 8 mon old and weighed. Weight and percentage in primal cuts and slaughter performance of Hanwoo and Holstein veal calves are obtained. Immediately after weighting, slices of loin and top round muscles were sampled. After vacuum packaging, the samples were subjected to proximate composition, physicochemical and microbiological analyses. Dressing weight and percentage were heavier and greater (p<0.05) in the Holstein than in the Hanwoo. Water contents of the top round muscle was higher in the Holstein than in the Hanwoo (p<0.05). Water-holding capacity, protein content and CIE L* (lightness) of both muscles were higher in the Holstein than in the Hanwoo veal, whereas fat content, pH, cooking loss, a* (redness), and b* (yellowness) were higher in the Hanwoo than in the Holstein veal (p<0.05). Thiobarbituric acid and volatile basic nitrogen values of both the muscles were lower in the Hanwoo than in the Holstein veal during the first 10 d of storage (p<0.05).

Introduction

Hanwoo may be originated from crossbreeding between Bos indicus in India and Bos primigenius in Europe and they have been reared for at least 2000 years in Korean peninsula (Han, 1996). Korean consumers prefer Hanwoo beef to imported beef, because they strongly believe Hanwoo beef have superior palatability characteristics (Hwang ). This kind of stereotype may not be easily broken, thereby Hanwoo have been maintained in superior position in meat quality in Korea (Hur ).

Holstein cattle are the premier dairy breed with a high potential for milk production (Jurie ). Therefore, Holstein breed have been introduced and raised as a domestic stock since 1903 in Korea (Cho ). In Korea, the statistics indicate that 64,107 Holstein beef were slaughtered and the frequencies of quality grading above grade 1 for Holstein steers were only 8.4% in 2013 (Korea Institute for Animal Products Quality Evaluation, 2015). Holstein beef have been not so popular and utilized limitedly because not only they have inferior palatability characteristics as compared to Hanwoo beef, but also their poor eating quality does not make it a viable choice for retail for Korean consumers. Some Holstein dairy farmers tried to produce the highly marbled Holstein steer by using a longer feeding period, but this was not financially advantageous for them, due to the expensive feeding cost and low feeding efficiency.

Traditionally, many European (Nederland, France, Switzerland, Italy, Belgium etc.) consumers demand veal because it has been regarded as the highest quality associated with a healthy product, low fat content, and a good smooth flavor (Vieira ). According to Council Regulation (EC) No 361/2008 of April 14th (EU, 2008), veal is described as the meat from unweaned calves that are slaughtered when they are no more than 8 mon old. The European Commission differentiates veal meat derived from calves of 16-19 wk of age (Ngapo and Gariépy, 2006). Presently, Korean consumes imported veal from Australia about 200 tons annually and have limitedly because of high price at the hotel restaurant and in-flight meals.

In Korea, Holstein cattle are slaughtered at an average age of 20-22 mon. Recently, young Holstein bulls have been problem for a livestock raiser in Korea. The farmers encounter serious challenges when they have new-born, male veal, given the unstable market price and low valuation of this product in the domestic beef market (Cho ). Most of the studies have focused on meat quality of Hanwoo. However, little research has been carried out on the meat quality of Holstein calves which was born and raised in Korea. Especially, the difference in meat quality of loin and top round of Hanwoo and Holstein veal has rarely been investigated. Therefore, the objective of the present work was to compare the physicochemical and microbial quality characteristics of loin (m. longissimus dorsi) and top round (m. semimembranosus) from the Hanwoo and Holstein veal.

Materials and Methods

Animals and slaughter procedure

A total of 5 young Hanwoo and 5 Holstein bull calves were reared at selected local cattle farm, where they were kept in individual boxes on slatted floors with individual bucket feeding. They were fed with breast milk during the first 8 wk. Calves remained with their mothers on natural pastures until weaning. After that, they were confined and fed with roughage and straw during 4-8 mon. Both calves were slaughtered at an average age of 8 mon and they were dressed in an officially approved slaughterhouse, according to standard methods, using a captive bolt stunner, followed by sticking and bleeding. The carcasses were immediately cooled at 0℃ for 24 h in a chilling room. Then, commercial fabrication procedures according to the guidelines of the notification of the Ministry of agriculture, food and rural affairs, the right side of each carcass was fabricated into bone-in primal cuts. Weight and percentage in primal cuts and slaughter performance of Hanwoo and Holstein veal calves are obtained. Immediately after weighting, slices of loin (m. longissimus dorsi) and top round (m. semimembranosus) muscles were taken. After vacuum packaged, the samples were transported to laboratory at university, South Korea. Immediately the samples were removed from vacuum packages. All subcutaneous fat and visible connective tissue of muscles were trimmed and re-vacuum packaged using vacuum package system (Vc999, K4N, Switzerland). Packaged samples were stored in refrigerator (CA-D17DC, LG, Korea) in which temperatures were controlled within 0±1℃ of designated storage temperature. These samples were analyzed at the 1, 3, 7, 10, 20 and 30 d for TBA, VBN and microbiological analyses.

Proximate composition

Immediately after keeping in a chilling room, samples from each treatment were analyzed for proximate composition. All determinations were carried out on the homogenized samples, in triplicate. Moisture, fat, protein and ash were determined on samples using with a slightly modified method of AOAC (2000).

Physico-chemical analyses

The pH values of samples were measured by blending a 10 g sample with 90 ml distilled water for 1 min in a homogenizer (Ultra-turrax, T25-S1, Germany). This analysis was determined by using a pH meter (PHM201, Radiometer, France). The water holding capacity (WHC) was conducted by a modification of the procedure of Grau and Hamm (1953). Three hundred mg sample of muscle was placed in a filter-press machine and compressed for 2 min. WHC was calculated from duplicate samples as a ratio of the meat film area to the total area; hence, a larger value suggests a higher WHC. WHC (%) was calculated as follows: WHC (%) = 100 − [total meat area/meat film area × 100]. For cooking loss, after the samples were thawed at 4℃ overnight before analyses and sliced with a thickness of 2 cm. The samples were weighed and cooked in an electric grill (EMG-533, AIJIA electric appliance, China) until they reached a final internal temperature of 70℃. Cooking loss was determined by the ratio of the difference between raw weight and final cooked weight as follows: Cooking loss (%) = 100 × (raw weight − final cooked weight) / raw weight. Shear force values were measured by the method described by the procedure of Bourne (1978). The samples were prepared a cubic form (30 × 30 × 20 mm) and six cores of 1.27 cm in diameter, were drilled parallel to the muscle fiber from each sample. Each core was sheared once with a Warner-Bratzler shear attachment using a texture analyzer (TA-XT2, Stable Micro System Ltd., U.K.). The maximum shear force value (kg) was recorded for each sample. Test and post-test speeds were set at 1.0 mm/s. Color measurements were taken using a Minolta chromameter (CR-410, Minolta Co. Ltd., Japan). CIE L*, a* and b* values were determined with measurements standardized with respect to a white calibration plate (L* = 94.4, a* = 0.313, b* = 0.319) at room temperature. Color measurements for each of three replicates, always trying to avoid area with excess fat were taken and the value was recorded. The TBARS of samples were analyzed by the modification method described by the procedure of Witte (1970). Readings were made on a spectrophotometer (X-MA 3000, Human Ltd., Korea) at 530 nm. A micro-diffusion method described by Conway (1950) was modified for the determination of volatile basic nitrogen (VBN) values in samples. Each sample (10 g) was homogenized (Ultra-turrax, T25-S1, IKA, Germany) for 1 min with 90 mL of distilled water. The supernatant solution was filtered using a filter paper (No. 4, Whatman). A 0.01 N of boric acid was placed in the inner section of a Conway micro-diffusion cell (Sibata Ltd., Japan). One ml sample solution and 1 mL of saturated K2CO3 were also placed into the outer section of the same cell, and the lid was immediately closed. The cell was incubated at 25℃ for 60 min, and it was then titrated against 0.02 N H2SO4. The VBN value was reported as mg/100g.

Microbiological analysis

Meats were subjected to microbiological analysis to monitor the dynamic changes in the populations responsible for the aging of the veal samples and their hygienic quality. The samples (10 g) were homogenized with 90 mL of 0.85% sterile peptone water using a Stomacher Lab blender (Interscience BagMixers, USA) for 2 min and serially diluted with saline solution by 10-fold. Total aerobic plate counts were enumerated on plate count agar (DifcoTM, Laboratories, USA) at 37℃ for 48 h. Bacterial counts were expressed as colony forming units per gram of sample (CFU/g).

Statistical methods

The experiment had three replications. Variance analysis (ANOVA) were performed on all the variables measured using the General Linear Model (GLM) procedure of the SAS statistical package (SAS, 2002). Duncan’s multiple range tests and t-test were used to determine differences among the treatment means. Mean values and deviations were reported. A probability level (p) of 0.05 was chosen as the limit for statistical significance in all tests.

Results and Discussion

Weight and percentage in primal cuts

Weight and percentage in primal cuts of Hanwoo and Holstein veal calves are presented in Table 1. Loin, strip loin, neck, and rib in primal cuts of the Hanwoo had higher weights and percentages than those of the Holstein. Ttenderloin, blade, topside, butt, brisket, and shank weights of the Holstein were higher than those of the Hanwoo. These results showed the presence of differences in the weight and percentage in primal cuts between Hanwoo and Holstein veal calves.

Table 1.

Weight and percentage in primal cuts of Hanwoo and Holstein veal calves

	Hanwoo		Holstein
	Weight (kg)	Percentage (%)	Weight (kg)	Percentage (%)
Tenderloin	2.46±0.01	2.76±0.01	2.76±0.02	2.92±0.02
Loin	12.70±0.10	14.22±0.19	10.14±0.07	10.72±0.89
Strip loin	3.50±0.10	3.92±0.20	2.88±0.09	3.05±0.05
Neck	7.02±0.20	7.86±0.42	6.60±0.20	6.98±0.11
Blade	8.87±0.11	9.33±0.22	10.14±0.10	10.72±0.95
Topside	9.88±0.10	11.07±0.23	10.32±0.09	10.91±0.93
Butt & rump	13.79±0.17	15.44±0.40	15.50±0.01	16.39±1.13
Brisket	10.51±0.31	11.77±0.71	17.60±4.47	18.14±7.31
Shank	6.76±0.01	7.57±0.04	7.88±0.01	8.33±0.59
Rib	13.80±0.73	15.45±1.60	11.20±0.51	11.84±1.03

Values are Mean±standard deviation (n=5).

Slaughter performance and proximate composition

As shown in Table 2, the Hanwoo calves were heavier (live weight of 280 kg) than the Holstein calves (live weight of 270 kg), but no difference was noted between the breeds. The carcass weights of the Hanwoo and Holstein veal calves were similar in this study. Dressing weight was higher and percentage was greater (p<0.05) in the Holstein than in the Hanwoo. Our results are supported by those of Gregory who showed that the breed of beef cattle could affect the dressing percentage.

Table 2.

Slaughter performance of Hanwoo and Holstein veal calves

	Hanwoo		Holstein
	Weight (kg)	Percentage (%)	Weight (kg)	Percentage (%)
Live weight at slaughter	280±10.50		270±11.43
Cold carcass weight	140±7.43		141±6.43
Dressing weight	76.8±0.63B		83.4±1.48A
Dressing percentage		54.9±0.1B		59.1±0.2A

A,BMeans with different superscripts in the same row are significantly different (p<0.05).

Values are Mean±standard deviation (n=5).

Comparison of the proximate compositions of loin (m. longissimus dorsi) and top round (m. semimembranosus) of Hanwoo and Holstein veal calves is shown in Table 3. Water content of the top round muscle was higher in the Holstein veal than in the Hanwoo sample (p<0.05). Fat and protein contents of both muscles differed significantly between the two breed groups (p<0.05). Hanwoo veal had higher fat content of both muscles, while Holstein veal had higher protein content. Ash contents were higher in the Holstein sample compared with Hanwoo; however, no significant differences were observed. A previous study (Hur ) found that water and protein contents were higher in the Holstein than in the Hanwoo. Our values are also similar to those for the loin and top round muscles of Holstein calves shown by Cho . Tuma showed that the m. longissimus dorsi from 6 mon-old calves contained 72.63% moisture, 21.24% protein, and 1.1% ash content. Generally, increase in the intramuscular fat content of the meat causes decrease in the water content (Varela, 2002), and these reports are consistent with our findings. High protein and low fat content of the loin and top round of Holstein veal calves were confirmed in our study.

Table 3.

Proximate composition of M. longissimus dorsi and semimembranosus in Hanwoo and Holstein veal

	Breed	Cut
		Longissimus dorsi	Semimembranosus
Water (%)	Hanwoo	73.49±0.30	73.76±0.50b
	Holstein	74.90±0.06	76.09±0.05a
Fat (%)	Hanwoo	1.92±0.39a	2.49±0.43a
	Holstein	0.65±0.16b	0.24±0.13b
Protein (%)	Hanwoo	21.77±0.12b	21.85±0.06b
	Holstein	24.21±0.20a	22.86±0.23a
Ash (%)	Hanwoo	1.10±0.05	1.15±0.09
	Holstein	1.17±0.06	1.37±0.03

a,bMeans with different superscripts in the same column are significantly different (p<0.05).

All values are mean±standard deviation (n=5).

Physicochemical traits

Some physicochemical properties of loin (m. longissimus dorsi) and top round (m. semimembranosus) in the Hanwoo and Holstein veal are presented in Table 4. Muscle pH values were higher in the Hanwoo than in the Holstein veal (p<0.05). Water-holding capacity (WHC) of both the muscles was significantly higher in the Holstein than in the Hanwoo veal, whereas cooking loss was higher in the Hanwoo veal (p<0.05). Sanudo also showed differences in the WHC among breeds. An inverse relationship is noted between moisture content and cooking loss (Jeremiah ). Futther, an inverse relationship is noted between WHC and fat content (Jeremiah ). As shown in Table 4, the shear force values of the loin muscle were lower in the Holstein than in the Hanwoo veal, which is similar to the results by Hur . Monteiro suggested that shear force in veal was more affected by cooking losses than by other physicochemical characteristics. Many studies have shown that shear force values are correlated with intramuscular fat content (Fiems ; Park ).

Table 4.

Physicochemical traits of M. longissimus dorsi and semimembranosus in Hanwoo and Holstein veal

	Breed	Cut
		Longissimus dorsi	Semimembranosus
pH	Hanwoo	6.05±0.01a	5.58±0.01a
	Holstein	4.72±0.01b	4.90±0.01b
WHC	Hanwoo	41.50±3.39b	39.26±9.81b
	Holstein	60.93±2.39a	58.72±4.44a
Cooking loss	Hanwoo	32.28±3.59a	35.85±6.35a
	Holstein	16.83±2.35b	20.83±4.12b
Shear force (kg)	Hanwoo	7.89±0.37a	10.63±0.60
	Holstein	4.39±0.29b	9.64±5.41
CIE L*	Hanwoo	39.65±2.19b	34.93±0.85b
	Holstein	45.54±0.11a	44.27±0.16a
CIE a*	Hanwoo	16.99±2.45a	18.68±3.14a
	Holstein	9.06±0.03b	8.67±0.05b
CIE b*	Hanwoo	8.45±1.56a	8.82±1.92a
	Holstein	2.41±0.03b	1.59±0.02b

a,bMeans with different superscripts in the same column are significantly different (p<0.05).

All values are mean±standard deviation (n=5).

Consumers typically assess veal quality based on the lean color (Ngapo and Gariépy, 2006). CIE L* (lightness) of both the muscles was significantly higher in the Holstein than in the Hanwoo veal. However, a* (redness) and b* (yellowness) of both the muscles were higher in the Hanwoo than in the Holstein veal (p<0.05), similar to the results of Hur , who reported higher a* and b* for Hanwoo than for Holstein veal. The veal industry depends strongly on lean color for carcass grading and determination of carcass value, as whiter graded carcasses command greater value (Cho ). The Holstein veal in this study showed a very light color, characterized by high L* values (45.54 for the loin and 44.27 for the top round) and low a* and b* values. Meat color depends on the concentration and oxidation state of myoglobin. Color changes are because of the formation of metamyoglobin, characteristic of the maturation process (Renerre, 1982). Color is also correlated with the pH, as lightness decreased with an increase in the pH (Guignot ). In addition, meat lightness (L*) could be affected by beef breed (Muchenje ).

Comparison of the changes in TBA, VBN, and total plate counts of m. longissimus dorsi and m. semimembranosus of the Hanwoo and Holstein veal during storage are shown in Table 5. TBA values of both the muscles were lower in the Hanwoo than in the Holstein veal during the first 10 d of storage (p<0.05). The initial value of the lipid oxidation in Holstein veal was about 0.3-0.4 mg/kg and remained unchanged for up to 30 d. On the other hands, oxidation levels in Hanwoo veal increased to approximately 0.8 mg/kg after 20 d. TBA values of all muscles were less than 1 mg malondialdehyde/kg, which is considered the limit of acceptability for rancidity for fresh meat (Ockerman, 1976). The VBN values of both muscles were lower in the Hanwoo than in the Holstein veal during the first 10 d of storage (p<0.05). Higher VBN values in the Holstein veal samples are due to the high protein content in this study. The VBN in Holstein meat can be explained by bacterial or enzymatic degradation of protein (Egan ). The VBN values of both breeds continuously increased during storage (p<0.05).

Table 5.

Change of TBA, VBN and total plate counts of M. longissimus dorsi and semimembranosus in Hanwoo and Holstein veal during storage

	Breed	Cut	Days
			1	3	7	10	20	30
TBA (mg malonaldehyde/kg)	Hanwoo	Longissimus dorsi	0.34bB	0.39bB	0.34bB	0.38bB	0.79aA	0.81aA
		Semimembranosus	0.31bC	0.39bB	0.37bB	0.35bB	0.44bA	0.45bA
	Holstein	Longissimus dorsi	0.49a	0.44a	0.48a	0.42a	0.44b	0.44b
		Semimembranosus	0.38b	0.38b	0.47a	0.42a	0.43b	0.43b
VBN	Hanwoo	Longissimus dorsi	10.28Cc	11.71cC	11.46cC	17.96bB	25.84aA	22.50bA
		Semimembranosus	12.55bB	13.85bB	14.01bB	18.64bB	17.35cB	25.61aA
	Holstein	Longissimus dorsi	14.89aB	16.57aB	17.62aB	22.02aA	23.30bA	22.48bA
		Semimembranosus	15.38aB	16.29aB	18.39aB	23.25aA	24.97aA	23.08bA
Total plate counts (Log CFU/g)	Hanwoo	Longissimus dorsi	2.44D	2.26D	4.07C	4.85B	6.06A	6.16A
		Semimembranosus	2.89C	2.56C	4.67B	6.08A	5.69A	6.30A
	Holstein	Longissimus dorsi	2.70C	2.56C	6.17B	6.17B	6.52B	6.92A
		Semimembranosus	2.91C	2.50C	5.47B	5.90A	6.13A	6.51A

1)Means of three replicate experiments with three samples analyzed per replicate (n=9).

a-cFigures with different letters within a same column differ significantly (p<0.05).

A-DFigures with different letters within a same row differ significantly (p<0.05).

The total aerobic counts of both breeds and all cuts tended to increase slowly during storage. The samples remained below the microbiological guidelines maximum limit (below 7 Log CFU/g) for meat as recommended by Korean Food Standards Codex (MFDS, 2015) for 30 d. Vacuum packaging retards microbiological growth, and delays the development of spoilage due to slow proliferation of bacteria capable of tolerating anaerobic conditions (Gill, 1992). Therefore, the shelf life of Holstein veal samples stored at 0℃ under vacuum conditions would be at least 30 d. Bacteria counts of veal appeared to be irrelevant in this study; breed and cut did not have an effect on bacteria counts of veal (p>0.05). This leads to the conclusion that veal shelf life could exceed 30 d under vacuum conditions.

Conclusions

Breed can affect the proximate composition and physicochemical traits of the Hanwoo and Holstein veal. In this study, Holstein veal had higher water, and protein contents, WHC, and L*, whereas Hanwoo veal had higher fat content, pH, cooking loss, shear force, a*, and b*. The results of this study provide objective information to consumers on relative meat quality of the Hanwoo and Holstein veal. Further, consumer preference toward Holstein veal could contribute to less demand for Hanwoo veal in the domestic beef market.