| Literature DB >> 26877223 |
Pratik A Lalit1, Max R Salick2, Daryl O Nelson3, Jayne M Squirrell4, Christina M Shafer5, Neel G Patel6, Imaan Saeed6, Eric G Schmuck7, Yogananda S Markandeya6, Rachel Wong6, Martin R Lea6, Kevin W Eliceiri4, Timothy A Hacker7, Wendy C Crone8, Michael Kyba9, Daniel J Garry10, Ron Stewart5, James A Thomson11, Karen M Downs3, Gary E Lyons3, Timothy J Kamp12.
Abstract
Several studies have reported reprogramming of fibroblasts into induced cardiomyocytes; however, reprogramming into proliferative induced cardiac progenitor cells (iCPCs) remains to be accomplished. Here we report that a combination of 11 or 5 cardiac factors along with canonical Wnt and JAK/STAT signaling reprogrammed adult mouse cardiac, lung, and tail tip fibroblasts into iCPCs. The iCPCs were cardiac mesoderm-restricted progenitors that could be expanded extensively while maintaining multipotency to differentiate into cardiomyocytes, smooth muscle cells, and endothelial cells in vitro. Moreover, iCPCs injected into the cardiac crescent of mouse embryos differentiated into cardiomyocytes. iCPCs transplanted into the post-myocardial infarction mouse heart improved survival and differentiated into cardiomyocytes, smooth muscle cells, and endothelial cells. Lineage reprogramming of adult somatic cells into iCPCs provides a scalable cell source for drug discovery, disease modeling, and cardiac regenerative therapy.Entities:
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Year: 2016 PMID： 26877223 PMCID： PMC4779406 DOI： 10.1016/j.stem.2015.12.001
Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633