Literature DB >> 26877024

Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis.

Manuel A Ortega1, Yue Hao1, Mark C Walker2, Stefano Donadio3, Margherita Sosio3, Satish K Nair1,4, Wilfred A van der Donk1,2.   

Abstract

Class I lantibiotic dehydratases dehydrate selected Ser/Thr residues of a precursor peptide. Recent studies demonstrated the requirement of glutamyl-tRNA(Glu) for Ser/Thr activation by one of these enzymes (NisB) from the Firmicute Lactococcus lactis. However, the generality of glutamyl-tRNA(Glu) usage and the tRNA specificity of lantibiotic dehydratases have not been established. Here we report the 2.7-Å resolution crystal structure, along with the glutamyl-tRNA(Glu) utilization of MibB, a lantibiotic dehydratase from the Actinobacterium Microbispora sp. 107891 involved in the biosynthesis of the clinical candidate NAI-107. Biochemical assays revealed nucleotides A73 and U72 within the tRNA(Glu) acceptor stem to be important for MibB glutamyl-tRNA(Glu) usage. Using this knowledge, an expression system for the production of NAI-107 analogs in Escherichia coli was developed, overcoming the inability of MibB to utilize E. coli tRNA(Glu). Our work provides evidence for a common tRNA(Glu)-dependent dehydration mechanism, paving the way for the characterization of lantibiotics from various phyla.
Copyright © 2016 Elsevier Ltd. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2016        PMID： 26877024      PMCID： PMC4798866          DOI： 10.1016/j.chembiol.2015.11.017

Source DB:  PubMed          Journal:  Cell Chem Biol        ISSN： 2451-9448            Impact factor:   8.116


  48 in total

1.  Structure and mechanism of the tRNA-dependent lantibiotic dehydratase NisB.

Authors:  Manuel A Ortega; Yue Hao; Qi Zhang; Mark C Walker; Wilfred A van der Donk; Satish K Nair
Journal:  Nature       Date:  2014-10-26       Impact factor: 49.962

2.  Family of class I lantibiotics from actinomycetes and improvement of their antibacterial activities.

Authors:  Sonia I Maffioli; Paolo Monciardini; Bruno Catacchio; Carlo Mazzetti; Daniela Münch; Cristina Brunati; Hans-Georg Sahl; Stefano Donadio
Journal:  ACS Chem Biol       Date:  2015-01-23       Impact factor: 5.100

3.  Microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes.

Authors:  Lucy C Foulston; Mervyn J Bibb
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-13       Impact factor: 11.205

4.  The discriminator base influences tRNA structure at the end of the acceptor stem and possibly its interaction with proteins.

Authors:  C P Lee; N Mandal; M R Dyson; U L RajBhandary
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-01       Impact factor: 11.205

5.  Expanded natural product diversity revealed by analysis of lanthipeptide-like gene clusters in actinobacteria.

Authors:  Qi Zhang; James R Doroghazi; Xiling Zhao; Mark C Walker; Wilfred A van der Donk
Journal:  Appl Environ Microbiol       Date:  2015-04-17       Impact factor: 4.792

6.  Evolution of lanthipeptide synthetases.

Authors:  Qi Zhang; Yi Yu; Juan E Vélasquez; Wilfred A van der Donk
Journal:  Proc Natl Acad Sci U S A       Date:  2012-10-15       Impact factor: 11.205

7.  Mechanistic dissection of the enzyme complexes involved in biosynthesis of lacticin 3147 and nisin.

Authors:  Anneke Kuipers; Jenny Meijer-Wierenga; Rick Rink; Leon D Kluskens; Gert N Moll
Journal:  Appl Environ Microbiol       Date:  2008-09-12       Impact factor: 4.792

8.  Identification of essential catalytic residues of the cyclase NisC involved in the biosynthesis of nisin.

Authors:  Bo Li; Wilfred A van der Donk
Journal:  J Biol Chem       Date:  2007-05-19       Impact factor: 5.157

9.  In silico analysis highlights the frequency and diversity of type 1 lantibiotic gene clusters in genome sequenced bacteria.

Authors:  Alan J Marsh; Orla O'Sullivan; R Paul Ross; Paul D Cotter; Colin Hill
Journal:  BMC Genomics       Date:  2010-11-30       Impact factor: 3.969

10.  Mechanistic studies on the substrate-tolerant lanthipeptide synthetase ProcM.

Authors:  Subha Mukherjee; Wilfred A van der Donk
Journal:  J Am Chem Soc       Date:  2014-07-08       Impact factor: 15.419
View more
  30 in total

1.  Investigation of Amide Bond Formation during Dehydrophos Biosynthesis.

Authors:  Emily C Ulrich; Despina J Bougioukou; Wilfred A van der Donk
Journal:  ACS Chem Biol       Date:  2018-01-12       Impact factor: 5.100

Review 2.  Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.

Authors:  Lindsay M Repka; Jonathan R Chekan; Satish K Nair; Wilfred A van der Donk
Journal:  Chem Rev       Date:  2017-01-30       Impact factor: 60.622

3.  O-Methyltransferase-Mediated Incorporation of a β-Amino Acid in Lanthipeptides.

Authors:  Jeella Z Acedo; Ian R Bothwell; Linna An; Abby Trouth; Clara Frazier; Wilfred A van der Donk
Journal:  J Am Chem Soc       Date:  2019-10-15       Impact factor: 15.419

4.  Structural Insights into Thioether Bond Formation in the Biosynthesis of Sactipeptides.

Authors:  Tyler L Grove; Paul M Himes; Sungwon Hwang; Hayretin Yumerefendi; Jeffrey B Bonanno; Brian Kuhlman; Steven C Almo; Albert A Bowers
Journal:  J Am Chem Soc       Date:  2017-08-21       Impact factor: 15.419

5.  Structures of the peptide-modifying radical SAM enzyme SuiB elucidate the basis of substrate recognition.

Authors:  Katherine M Davis; Kelsey R Schramma; William A Hansen; John P Bacik; Sagar D Khare; Mohammad R Seyedsayamdost; Nozomi Ando
Journal:  Proc Natl Acad Sci U S A       Date:  2017-09-11       Impact factor: 11.205

6.  Assessing the Flexibility of the Prochlorosin 2.8 Scaffold for Bioengineering Applications.

Authors:  Julian D Hegemann; Silvia C Bobeica; Mark C Walker; Ian R Bothwell; Wilfred A van der Donk
Journal:  ACS Synth Biol       Date:  2019-05-08       Impact factor: 5.110

7.  Characterization of glutamyl-tRNA-dependent dehydratases using nonreactive substrate mimics.

Authors:  Ian R Bothwell; Dillon P Cogan; Terry Kim; Christopher J Reinhardt; Wilfred A van der Donk; Satish K Nair
Journal:  Proc Natl Acad Sci U S A       Date:  2019-08-13       Impact factor: 11.205

8.  Characterization of Leader Peptide Binding During Catalysis by the Nisin Dehydratase NisB.

Authors:  Lindsay M Repka; Kenton J Hetrick; See Hyun Chee; Wilfred A van der Donk
Journal:  J Am Chem Soc       Date:  2018-03-20       Impact factor: 15.419

Review 9.  Cameo appearances of aminoacyl-tRNA in natural product biosynthesis.

Authors:  Emily C Ulrich; Wilfred A van der Donk
Journal:  Curr Opin Chem Biol       Date:  2016-09-04       Impact factor: 8.822

10.  Structural insights into enzymatic [4+2] aza-cycloaddition in thiopeptide antibiotic biosynthesis.

Authors:  Dillon P Cogan; Graham A Hudson; Zhengan Zhang; Taras V Pogorelov; Wilfred A van der Donk; Douglas A Mitchell; Satish K Nair
Journal:  Proc Natl Acad Sci U S A       Date:  2017-11-20       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.