Helen Williams1,2, Gabriel Cassorla3, Nicholas Pertsoulis3, Vyoma Patel3, Mauro Vicaretti3,4, Najwa Marmash5, Kerry Hitos6, John P Fletcher3,4, Heather J Medbury3,4. 1. Vascular Biology Research Centre, Department of Surgery, Westmead Hospital, Westmead, Australia - helen.williams@sydney.edu.au. 2. Sydney Medical School, University of Sydney, Westmead, Australia - helen.williams@sydney.edu.au. 3. Vascular Biology Research Centre, Department of Surgery, Westmead Hospital, Westmead, Australia. 4. Sydney Medical School, University of Sydney, Westmead, Australia. 5. Research and Education Network, WSLHD, Westmead Hospital, Westmead, Australia. 6. Westmead Research Centre for Evaluation of Surgical Outcomes, Department of Surgery, University of Sydney, Westmead Hospital, Westmead, Australia.
Abstract
BACKGROUND: Specific monocyte and macrophage subsets have been implicated in atherosclerosis, with intermediate monocytes proportionally elevated in cardiovascular disease and M1 macrophages abundant in unstable atherosclerotic plaques. While several studies have shown altered proportions of these subsets in atherosclerosis, studies examining functional and phenotypic subset alterations remain scarce. METHODS: We used whole blood flow cytometry to investigate the expression of M1 (CD86) and M2 (CD163) markers on monocyte subsets of atherosclerotic patients and controls. RESULTS: Atherosclerotic patients had a more inflammatory monocyte profile than controls, indicated by increased intermediate subset proportions, a higher classical monocyte CD86/CD163 ratio, and elevated serum M1-related chemokines. A more inflammatory profile appeared to correlate with atherosclerotic risk, as in controls classical monocyte CD86/CD163 ratio was negatively correlated with HDL and apolipoprotein A1, and positively correlated with interleukin-1β. CONCLUSIONS: We conclude that monocyte subsets show functional and phenotypic changes in cardiovascular disease and such changes are likely to contribute to atherosclerotic progression.
BACKGROUND: Specific monocyte and macrophage subsets have been implicated in atherosclerosis, with intermediate monocytes proportionally elevated in cardiovascular disease and M1 macrophages abundant in unstable atherosclerotic plaques. While several studies have shown altered proportions of these subsets in atherosclerosis, studies examining functional and phenotypic subset alterations remain scarce. METHODS: We used whole blood flow cytometry to investigate the expression of M1 (CD86) and M2 (CD163) markers on monocyte subsets of atheroscleroticpatients and controls. RESULTS:Atheroscleroticpatients had a more inflammatory monocyte profile than controls, indicated by increased intermediate subset proportions, a higher classical monocyte CD86/CD163 ratio, and elevated serum M1-related chemokines. A more inflammatory profile appeared to correlate with atherosclerotic risk, as in controls classical monocyte CD86/CD163 ratio was negatively correlated with HDL and apolipoprotein A1, and positively correlated with interleukin-1β. CONCLUSIONS: We conclude that monocyte subsets show functional and phenotypic changes in cardiovascular disease and such changes are likely to contribute to atherosclerotic progression.
Authors: Maciej M Kowalik; Piotr Trzonkowski; Magdalena Łasińska-Kowara; Andrzej Mital; Tomasz Smiatacz; Miłosz Jaguszewski Journal: Cardiol J Date: 2020-05-07 Impact factor: 2.737
Authors: Rekha Marimuthu; Habib Francis; Suat Dervish; Stephen C H Li; Heather Medbury; Helen Williams Journal: J Vis Exp Date: 2018-10-17 Impact factor: 1.355
Authors: Vyoma K Patel; Helen Williams; Stephen C H Li; John P Fletcher; Heather J Medbury Journal: Front Immunol Date: 2021-02-26 Impact factor: 7.561