Literature DB >> 2687117

Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae.

E Kanaya1, T Higashizaki, F Ozawa, K Hirai, M Nishizawa, M Tokunaga, H Tsukui, H Hatanaka, F Hishinuma.   

Abstract

The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast alpha-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2687117     DOI： 10.1016/0378-1119(89)90404-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  7 in total

1.  Recombinant human nerve growth factor with a marked activity in vitro and in vivo.

Authors:  Anna M Colangelo; Nicoletta Finotti; Michela Ceriani; Lilia Alberghina; Enzo Martegani; Luigi Aloe; Laura Lenzi; Rita Levi-Montalcini
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-20       Impact factor: 11.205

2.  Molecular analysis of POP2 gene, a gene required for glucose-derepression of gene expression in Saccharomyces cerevisiae.

Authors:  A Sakai; T Chibazakura; Y Shimizu; F Hishinuma
Journal:  Nucleic Acids Res       Date:  1992-12-11       Impact factor: 16.971

3.  Biologically active human and mouse nerve growth factors secreted by the yeast Saccharomyces cerevisiae.

Authors:  M Nishizawa; F Ozawa; T Higashizaki; K Hirai; F Hishinuma
Journal:  Appl Microbiol Biotechnol       Date:  1993-02       Impact factor: 4.813

4.  Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty.

Authors:  A Sakai; Y Shimizu; F Hishinuma
Journal:  Appl Microbiol Biotechnol       Date:  1990-06       Impact factor: 4.813

5.  Functional analysis of the threonine- and serine-rich Gp-I domain of glucoamylase I from Aspergillus awamori var. kawachi.

Authors:  T Semimaru; M Goto; K Furukawa; S Hayashida
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792

6.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-02-11       Impact factor: 16.971

7.  EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae.

Authors:  Jérôme Maury; Susanne M Germann; Simo Abdessamad Baallal Jacobsen; Niels B Jensen; Kanchana R Kildegaard; Markus J Herrgård; Konstantin Schneider; Anna Koza; Jochen Forster; Jens Nielsen; Irina Borodina
Journal:  PLoS One       Date:  2016-03-02       Impact factor: 3.240
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.