Saime Sezer1, Semiha Kurt2, Omer Ates3. 1. Department of Medical Biology, Gaziosmanpasa University, Tokat, Turkey. Electronic address: saimesezer@gmail.com. 2. Department ofNeurology, Medical Faculty, Gaziosmanpasa University, Tokat, Turkey. 3. Department of Medical Biology, Gaziosmanpasa University, Tokat, Turkey.
Abstract
BACKGROUND: Migraine is a complex neurological disorder characterized by severe recurrent headache, nausea, vomiting, photophobia, and phonophobia. The frequency and duration of these symptoms varies among individuals. Dopaminergic systems are believed to be involved in migraine pathophysiology. We aimed to look for association of polymorphisms in dopaminergic genes in genetic susceptibility to migraine in Turkey population. METHODS: The present study was designed to explore possible association of three polymorphisms, (1021C>T (Rs1611115), +1603C>T (Rs6271; C535R) and +444G>A (rs1108580), of Dopamin Beta Hydroxylase gene in migraine patients. 200 migraine patients and 267 healthy controls were included in the study. Genomic DNA was extracted from blood and genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism methods (PCR-RFLP). RESULTS: Statistical evaluation of data results showed a significant association for allelic and genotypic frequency distribution between the Dopamin Beta Hydroxylase gene +1603C>T polymorphism and migraine (p=0.000, OR: 4.36, 95% CI: 2.73-7.16). There was no association observed between the -1021C>T and +444 G>A polymorphisms of the Dopamin Beta Hydroxylase gene and migraine (p=0.8731 and p=0.7584). CONCLUSIONS: This study reflects that Dopamin Beta Hydroxylase gene +1603C>T polymorphism may be one of the many genetic factors for migraine susceptibility in the Turkish population.
BACKGROUND:Migraine is a complex neurological disorder characterized by severe recurrent headache, nausea, vomiting, photophobia, and phonophobia. The frequency and duration of these symptoms varies among individuals. Dopaminergic systems are believed to be involved in migraine pathophysiology. We aimed to look for association of polymorphisms in dopaminergic genes in genetic susceptibility to migraine in Turkey population. METHODS: The present study was designed to explore possible association of three polymorphisms, (1021C>T (Rs1611115), +1603C>T (Rs6271; C535R) and +444G>A (rs1108580), of Dopamin Beta Hydroxylase gene in migrainepatients. 200 migrainepatients and 267 healthy controls were included in the study. Genomic DNA was extracted from blood and genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism methods (PCR-RFLP). RESULTS: Statistical evaluation of data results showed a significant association for allelic and genotypic frequency distribution between the Dopamin Beta Hydroxylase gene +1603C>T polymorphism and migraine (p=0.000, OR: 4.36, 95% CI: 2.73-7.16). There was no association observed between the -1021C>T and +444 G>A polymorphisms of the Dopamin Beta Hydroxylase gene and migraine (p=0.8731 and p=0.7584). CONCLUSIONS: This study reflects that Dopamin Beta Hydroxylase gene +1603C>T polymorphism may be one of the many genetic factors for migraine susceptibility in the Turkish population.