| Literature DB >> 26867633 |
Kei Hiruma1, Yusuke Saijo2,3.
Abstract
After Colletotrichum storage methods in Chapter 23 , we describe here experimental methods for the inoculation of Colletotrichum higginsianum (C.h.) on Arabidopsis leaves. We put a particular focus on the methods for lesion measurements after the drop-inoculation of the leaves and on C.h. biomass measurements in the leaves by RT-qPCR analysis. As an option, we also briefly describe methods for counting C.h. entry ratio.Entities:
Keywords: Arabidopsis; Biomass measurements; Colletotrichum higginsianum; Lesion measurements; RT-qPCR
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Year: 2016 PMID： 26867633 DOI： 10.1007/978-1-4939-3356-3_24
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745