Virus-Induced Gene Silencing for Gene Function Studies in Barley.

Virus-Induced Gene Silencing (VIGS) creates a natural antiviral defense in plants. However, it has been also a powerful tool for endogenous gene silencing in dicot and monocot plants by exploitation of recombinant viruses, harboring silencing inducing sequences. The Barley Stripe Mosaic Virus (BSMV) based VIGS system is an efficient and rapid RNAi approach that is routinely applied in functional genomics studies of cereals. We present here a protocol for BSMV VIGS application in barley based on mechanical inoculation of the plants with in vitro transcribed recombinant BSMV RNAs as the silencing triggers.