Literature DB >> 26862555

Lipidomic dataset of plasma from patients infected with wild type and nef-deficient HIV-1 strain.

Peter Meikle1, Hann Low1, Melissa J Churchill2, Michael Bukrinsky3, Dmitri Sviridov1.   

Abstract

Previous in vitro and in vivo studies demonstrated that HIV protein nef plays a key role in impairing cellular and systemic cholesterol metabolism in HIV disease, but clinical support for these findings is lacking. Here we present the data of comparative lipidomic analysis (330 lipid species) of plasma samples from HIV-negative subjects, patients infected with WT HIV-1 strain and patients infected with nef-deficient strain of HIV-1. We determine which effects of HIV on plasma lipidome are explained by the presence of nef. The data can be used to evaluate cardiovascular risk in HIV disease and to assess the role of nef in HIV-induced disturbances in systemic lipid metabolism. The full impact of nef deficiency on lipid and lipoprotein metabolism in HIV-infected patients is presented in the accompanying study "Lipid Metabolism in Patients Infected with Nef-deficient HIV-1 Strain" [1].

Entities:  

Year:  2015        PMID： 26862555      PMCID： PMC4706608          DOI： 10.1016/j.dib.2015.11.067

Source DB:  PubMed          Journal:  Data Brief        ISSN： 2352-3409


Specifications Table Value of the data The data can be used to assess the effect of HIV disease on lipid metabolism. The data can be used to assess the role of HIV nef in modifications of lipid metabolism in patients with HIV disease. The data can be used to evaluate cardiovascular risk in HIV disease.

Data

Table 1 shows concentrations of the individual lipid species (lipidomic analysis) in plasma samples from HIV-negative subjects, patients infected with WT HIV-1 strain and patients infected with nef-deficient strain of HIV-1.
Table 1

Abundance of lipid species in patient plasma.

Lipid SpeciesHIV NegativeWT HIVHIV (∆Nef)
dhCer 16:046.3±6.671.5±15.7⁎120.7±70.4⁎⁎
dhCer 18:046.1±11.379.5±22⁎194.1±184.3⁎
dhCer 20:020.8±5.623.4±9.1115.7±125.8⁎
dhCer 22:089.9±18.6125±44.4274.2±187.3
dhCer 24:0207.2±42.7260.1±98.7470.1±288.5
dhCer 24:1108.6±31153±42.8264.1±197.2
Cer 16:0216.9±20.5246.3±55.8344.7±171
Cer 18:080.4±16102.9±26.6140.7±76.9⁎
Cer 20:084.7±17.886.2±15.4166.2±122.2
Cer 22:0663.2±67.8617.6±84.4723.6±266.5
Cer 24:02647.7±320.92155.3±350.62014.1±684.1
Cer 24:11089.8±474.5963.3±253.2862±225.7
MHC 16:01285.4±3081618.9±471.61402.1±398.4
MHC 18:0252±77.1231.8±41.4270.3±67.3
MHC 20:0259.7±83.5230.8±48.8278.3±106.6
MHC 22:02758.9±769.82474.4±705.92608.6±1102.7
MHC 24:03975.1±1330.23863.1±1217.43821.1±1177.1
MHC 24:13203.8±1586.73114.6±883.12508.1±899.7
DHC 16:06594±13875239.7±1178.65250.8±1168.1
DHC 18:0117.6±20.7115.1±26.7159.7±79.3
DHC 20:0111.2±38.385±38.6256.2±249.5#
DHC 22:0520.3±182.7368.2±118.41128.3±1010.9
DHC 24:0540.2±183.4373.3±111888±659.1
DHC 24:12043.7±630.51372.2±370.91522.4±534.9
THC 16:01250.3±153.91252±311.61344.5±437.2
THC 18:0203.2±49.4178.9±56.8257.3±106.9
THC 20:067.1±6.555.7±15.9139.3±116
THC 22:0289.5±41243.6±49687.3±694.6
THC 24:0350.7±54.1301.5±68.5615.5±505.2
THC 24:1698.6±71643.6±166.5953.8±653.3
GM3 16:01186.8±142.41499.7±503.61250.2±314.1
GM3 18:0794.1±125.8748.6±195.3791.6±191.6
GM3 20:0361.1±94.8353.9±72.9444.7±134.2
GM3 22:01177±273.11137.9±223.41274.8±443.1
GM3 24:01179.9±286.4928.2±2041012.7±180.2
GM3 24:11918.4±1014.51692±532.31581.7±471.8
SM 31:1347±110.5316.1±60.8385.2±149.5
SM 32:0423±84.8512±136.4666.2±176⁎
SM 32:110970.2±2867.311084.2±1986.612645.4±3860.1
SM 32:2868.4±175.4828.5±196.6961.5±461
SM 33:16931.2±1781.17052.7±1579.67729.3±2074.5
SM 34:04369±686.25402.1±1442.66326±803.1⁎⁎
SM 34:1120736.7±12384.6129323.9±26176.6129129.6±22409.9
SM 34:215620.5±2496.815680.2±129416322.5±5561.5
SM 34:3135.9±24.5124.9±19163.3±98.7
SM 35:14815.2±876.84845.4±1167.25248.6±1542.4
SM 35:2773.9±190.4705.4±185.2781.8±310.9
SM 36:122607±3503.325721.4±3228.325610.4±5220.1
SM 36:29749.1±1996.59579.6±1292.410097.5±3951.9
SM 36:31074.4±360.1924±190.81074±643.2
SM 37:2512.7±192.1500.2±173.8521.5±291.6
SM 38:120644±4108.919264.3±2275.621268.4±5843.8
SM 38:26799.4±1206.86278.9±773.26563.5±1873.8
SM 39:110157.8±2045.27490.5±1875.67619.5±2037.8
SM 41:118996±4036.717979.3±2004.917306.7±6331.9
SM 41:219727.3±3049.417171.9±2962.816034.9±5041.4
SM 42:126934.6±4203.425312±510026693.5±8431
PC 28:0368.6±286.4376.4±250.1435.5±168.8
PC 29:091.2±48.695.1±54.7116.8±62.2
PC 30:04134.8±2489.44184.1±17464956.2±2064.6
PC 31:11389.3±208.71454.6±267.71555.7±508
PC 31:0979.1±388.11054.1±406.61209.8±563.1
PC 32:3458.5±86.5376.4±85.7421±155.2
PC 32:27219.7±1407.86448.7±1665.96654.3±2050.4
PC 32:129454.1±25941.637082.1±2345530935.6±15626
PC 32:012671.1±2388.313403.8±2964.817114.9±6334
PC 33:35682.1±1553.14107.9±708.24656.7±1065.8
PC 33:24583.7±1419.73659.3±1057.23564.5±1192.7
PC 33:14384.5±1011.45046±1359.24481.8±1552
PC 33:01655.6±348.41631.8±314.11916.6±510.2
PC 34:5171.6±70.9229.6±186.5240.4±98.6
PC 34:41606.8±581.81652.5±786.61834.3±717
PC 34:322769.1±735219858.9±5837.620107.6±5685.3
PC 34:2321046.6±34684290899.5±22001.8293708.3±41432.2
PC 34:1195585.8±52480.2206850.7±55249.4197388.1±53998.2
PC 34:04348.4±721.14120.7±721.35802.8±2909.4
PC 35:5113.2±51.2157.6±72.2169.3±75.2
PC 35:41240.9±652.11203±432.61246.2±501.3
PC 35:31901.1±134.71581.4±501.91506.1±592.2
PC 35:213544.2±2490.19696.8±2595.710090.5±2772.5
PC 35:18526.9±1780.97819.8±18727446.2±1690.3
PC 35:0320.7±67.9281±72.2391.4±120.7
PC 36:6861.1±256.21095.5±981.71001.5±345.8
PC 36:525911.8±9200.727956.2±10197.138102.1±10058.6
PC(16:0/20:4)112686.7±26551.2121232.6±21119.6127767.4±32566.9
PC(18:1/18:3)112686.7±26551.2121232.6±21119.6127767.4±32566.9
PC 36:3152274±15318.6142470.1±19125.1143953.6±22671
PC 36:2252660.6±29711.9210302±11360.1218774.2±27341.8
PC 36:165109.3±34001.460096.2±11547.466580.9±27261.1
PC 36:0406.3±101301.9±56.4791±632.5
PC 37:6797.3±362.2743.9±118.1698±223.9
PC 37:5997.2±204.11155±4451190.7±384.4
PC 37:46743.4±2109.55567.9±1941.85887.4±1628.4
PC 38:72140.7±487.22072.9±1360.12200.9±673.7
PC(16:0/22:6)51011.5±10081.253855.3±21311.452577.1±9403.7
PC(18:2/20:4)7393.4±15106016.4±1803.36887.3±2773.6
PC 38:559549.9±1464357159.2±11130.564488.3±12874.4
PC 38:4114510.3±30187.9103817.1±15881.4117427.7±34441.3
PC 38:350030.3±10091.254515.2±17004.952342.7±17609.6
PC 38:234797.2±6120.133767.7±4012.433369.1±6714
PC 39:783±16.766.9±27.270.3±19.5
PC 39:62128±631.71710.6±254.61721±573
PC 39:51169.4±171.9958.2±154.9993.3±286.5
PC 40:81759.1±159.31433±347.51964.9±654.6
PC 40:76129.3±1808.94631.1±1161.65225.3±1183.4
PC 40:625202.8±5582.428273.7±8375.129250.9±13740.5
PC 40:519341.9±7261.418482.1±3718.421315±7927.1
PC 40:43645.4±1483.43399±698.94450.1±2350.6
PC 14:0_0:01450.5±967.41648.7±700.82068.5±1021.5
PC 15:0_0:0712.4±188.1909.3±398.6862.4±378.9
PC 16:0_0:064035.6±16477.781270.5±24156.479525±28789.3
PC 16:1_0:03073.1±3006.73838.9±2309.43347.7±1738.2
PC 17:0_0:01733±3041781.3±5981721.4±591.5
PC 17:1_0:0412.9±226.4475.9±140.3447.3±197.6
PC 18:0_0:020686.9±489222679±4473.824098.5±8511.9
PC 18:1_0:020484.1±12961.719506.7±7474.216096.6±5343.9
PC 18:2_0:025858.1±3718.120702.9±4240.816748.8±5693.6
PC 18:3_0:0878.5±600.8686.3±236.6620.4±268.8
PC 20:0_0:0140.6±47.7108±20.6160.9±74
PC 20:1_0:0284.4±142.5252.4±75.3357.9±153.6
PC 20:2_0:0161.7±74.9136.2±54.8155.3±60.2
PC 20:3_0:02678.8±1133.12945.5±1104.92085.1±1098.2
PC 20:4_0:05258.2±1461.75528.7±1435.14635.1±2121.9
PC 20:5_0:0712.6±301.91214.2±1064.5900.4±451
PC 22:0_0:033.8±4.525±5.228±10
PC 22:1_0:020.8±10.519.3±5.223.3±8.2
PC 22:5_0:0693.9±404642.1±211561.9±224.4
PC 22:6_0:01521.4±489.91750.5±1071.81209.3±335.3
PC 24:0_0:073±10.655±1756.3±11.4
PC 26:0_0:018.6±3.315.7±6.816.1±3.1
PC(O-32:0)1760.1±422.51725.1±264.11765±369.2
PC(O-32:1)536.6±129495.5±180.3476.9±140.8
PC(O-32:2)99.9±43.6112.8±77.3126.3±67.5
PC(O-34:1)4309.9±585.33993.5±518.94198.4±983
PC(O-34:2)4254.6±1277.43382.8±360.23632±916.7
PC(O-34:3)123.9±19.796.6±20.8117.2±49
PC(O-34:4)263±28.9232.9±90.6254.6±114.7
PC(O-35:4)137.1±46.9168.3±62.8172.6±101
PC(O-36:0)62.2±18.242.6±10.1164.9±154.3
PC(O-36:1)699.4±170.2550.8±92673±284.1
PC(O-36:2)2607.4±615.21795.9±208.62113±596
PC(O-36:3)4460.3±850.74223.1±270.74185.6±869.3
PC(O-36:4)11578.9±3052.712290.3±1957.412383.1±3596.9
PC(O-36:5)568.1±195.7885.1±499.9899.8±360.8
PC(O-38:4)8185.4±2462.88238.8±809.68352.4±2172.1
PC(O-38:5)10828.4±2246.110739.9±916.411138.4±2171.4
PC(O-40:5)2945.4±478.92783.5±435.72689.5±647.2
PC(O-40:6)2031.5±445.62113.1±407.92130.7±363.3
PC(O-40:7)1698.3±431.31701.5±4401721.2±362.3
PC(P-30:0)122.5±56.5107.8±36.8116.1±18.3
PC(P-32:0)1330.4±440.41312.3±2301423±230.3
PC(P-32:1)225±73.5225.4±90.2245.9±85.2
PC(P-34:1)2383±276.42218.2±425.32474.3±417.2
PC(P-34:2)5682.1±1553.14107.9±708.24656.7±1065.8
PC(P-34:3)87.3±14.488.2±19.8113±41.4
PC(P-36:2)2427.2±6751886.1±418.22214.6±479.6
PC(P-36:4)7145.8±7317776.4±16918379.8±2858
PC(P-36:5)576.1±118.2827.7±441.2958.4±574.4
PC(P-38:4)2954.2±435.33072.1±540.93339.6±1290.2
PC(P-38:5)4598.9±761.85059.4±1125.25301.6±1374.1
PC(P-38:6)872.6±173.21079.8±468.51059±319.5
PC(P-40:6)482.8±143.1585.9±260.4559.9±156.8
PC(O-16:0/0:0)313±86.8389.6±82.1491.2±207.2
PC(O-18:0/0:0)102.6±27.3107.9±24.6163.9±87.8
PC(O-18:1/0:0)229.9±89.1246.8±49.7361.3±151.8
PC(O-20:1/0:0)16.7±7.613.7±3.917.9±8.6
PC(O-22:0/0:0)42.2±11.542±7.836.3±5
PC(O-22:1/0:0)36.1±16.428±823.6±7.3
PC(O-24:0/0:0)83.9±27.982.3±2179.2±6
PC(O-24:1/0:0)120.2±62.294.7±27.185.2±13.8
PC(O-24:2/0:0)20.8±8.511.4±413.2±2.9
PE 32:1112.3±173.3136.9±140.1147.1±151.1
PE 34:11786.3±2028.91667.2±896.32553.2±2061.4
PE 34:22699.6±1330.12753.3±12123475.8±1896.7
PE 34:3224.1±197.4184.7±101.7230.4±146.8
PE 35:1161.8±146.1136.7±60.1170±89.9
PE 35:2184.5±99.1146.9±76.7174.3±67.8
PE 36:12235.9±2702.61328.5±3682849±2193.8
PE 36:29985.6±7296.16783.9±1663.911031.4±6448
PE 36:33153±2349.31952.6±598.73443.1±1787.9
PE 36:42838.8±1220.92853.6±1199.94855.5±3665.1
PE 36:5293.3±277.9367.5±392.9414.9±270.7
PE 38:31769.5±1145.21402.2±612.92760.4±2211.3
PE 38:48155.7±3969.96235.9±1782.515795.8±13903.8
PE 38:53251.4±2184.12955±1455.25273.9±3989.3
PE 38:63358.7±1034.74436.2±2952.14777.5±3375.1
PE 40:4231.2±212.1129±40.9776.9±873.6
PE 40:5845±626.1772.3±285.51573.2±1389
PE 40:61985.1±1012.52217.1±989.52780±2053.9
PE 40:7390.9±251.1314.6±173.2537±386.4
PE(O-18:0/22:5)189.5±38.7201.9±68.7224.1±97.6
PE(O-18:1/18:2)170.6±46.1173.6±71.8134.4±45.7
PE(O-18:1/20:3)576.3±102.3646.5±208.9851±409.8
PE(O-18:2/18:2)600.3±127.1762.9±221.5734.7±287.9
PE(O-18:2/20:3)823.9±73.2926.8±336.4887.4±303.9
PE(O-18:2/22:5)213.8±28.5226±92.4241.5±71.4
PE(O-34:1)152.2±31.8147.1±67.8139.8±41.8
PE(O-34:2)92.1±35.989.4±35.578±32.8
PE(O-36:2)70.5±16.788.7±39.986±39.5
PE(O-36:5)65.7±1493.1±72.391.4±65.1
PE(O-36:6)34.3±10.471.7±44.995.4±32.5⁎⁎
PE(O-40:6)210.2±24.8210.4±52.8208.9±86.2
PE(P-16:0/18:1)66.7±12.884.4±38.6107.8±60.8
PE(P-16:0/18:2)100.6±40.4135.4±50.3127.9±27.1
PE(P-16:0/20:4)331.1±19.9409.4±122.2951.1±754.5
PE(P-16:0/22:5)1032.9±105.81382.6±732.51628±691.8
PE(P-16:0/22:6)273.1±59.2353.6±172.7374.1±76.2
PE(P-18:0/18:1)70.5±16.788.7±39.986±39.5
PE(P-18:0/18:2)311.5±118.8337.1±104.9329.7±73.2
PE(P-18:0/20:4)737±81799.3±260.21987.7±1556.7
PE(P-18:0/22:5)395.8±50.8449.6±258.8627.6±322.6
PE(P-18:0/22:6)219.4±36.2286.2±197.7286.2±77.5
PE(16:0_0:0)1355.4±601.21827.6±933.31669.1±711.2
PE(18:0_0:0)2434.3±1267.82559.4±8523768.1±2358.8
PE(18:1_0:0)3437.2±3836.12205.6±691.92285±847.4
PE(18:2_0:0)4242±21153335.9±937.72380.2±564.1
PE(20:4_0:0)1891.9±667.51702.2±298.41576.4±452
PE(22:6_0:0)1377.9±402.51723.5±836.71042.7±168.4##
PI 32:0384.5±238.91302.4±1204.31240.2±1080.8
PI 32:1819.7±771.73314.5±3854.22080.4±1952.6
PI 34:0153.5±58362.2±298.8⁎342.8±287.7
PI 34:18427±4815.117398.1±11562.112850.4±9325.1
PI 36:19148.6±4428.311986.8±4399.99382.8±4817.2
PI 36:222965.5±6974.425557.5±8566.820820±3299.5
PI 36:36773.6±3294.26093.6±1601.86415.4±1885.4
PI 36:46193.6±2762.26555.4±2189.828562.8±50448.9
PI 38:2775.6±536540±103.1738.7±412.5
PI 38:311259.1±4650.48023.3±1480.19582.2±2559.5
PI 38:442280.7±11985.928710.8±5496.647736.2±21064.5
PI 38:54390.8±3078.93827.9±1264.15561.7±2560.2
PI 38:6901.9±428.91641.8±1327.51364.7±561.9
PI 40:4559.6±250.2515.2±62.7804.8±532.7
PI 40:51608.8±583.22479±935.92218.6±988.7
PI 40:61647±616.12981.7±2190.12165.5±928.8
PI (18:0_0:0)707.4±218.5700.5±143.11378.7±872.5
PI (18:1_0:0)490.2±206.2710.8±385.3634.2±284.8
PI (18:2_0:0)495.9±156.9692.2±365.4494.9±192.9
PI (20:4_0:0)650.2±161.5589±126.1629.7±142.3
PS 36:11418.5±935.8⁎⁎259.4±259.97733.4±9425.3#
PS 38:3299.8±158.4⁎53.7±91.91559.3±1791.7#
PS 38:4191.7±133.1135.7±62.2316.2±244.8
PS 40:5123±65.4⁎⁎13.6±33.4590.9±659.3#
PS 40:6128.3±53.936.5±49.6572.1±619.6#
PG 18:0_18:1110.6±101.976.4±26.9105.7±68.8
PG 18:1_18:180.4±71.666.1±26.688.1±55.1
CE 14:019911.2±15468.121884.7±9734.923811.5±9856.2
CE 15:017699.3±5671.722221.3±1081820997.5±6853.8
CE 16:0318876.5±42174.4365155.6±30084.7353655.5±52830.9
CE 16:1115561.6±152024.7151872.5±70309.1134746.3±92283.7
CE 16:22779.4±1580.83445.8±1357.53464.8±1066.5
CE 17:08220.8±1483.99469.3±4026.27904.5±2728.8
CE 17:134230.6±810342325.3±9076.738586.9±7584.2
CE 18:017830.9±2511.820183.7±6264.915798.2±5758.3
CE 18:1300937±93437.4304312.4±43611.3281342.1±59416.8
CE 18:2318759.8±45580.4350004.5±54887329663.9±68020.6
CE 18:353088.2±26201.857313.5±16055.854718.4±19826.1
CE 20:1272.6±60.2255.9±71.9225.5±45.5
CE 20:21476.3±554.31848.2±545.91611.8±644.6
CE 20:321197.7±6332.222717.8±466021939.8±7333.3
CE 20:4125537.8±42656.5145713.5±27527.5139683.5±54248.4
CE 20:534335.7±15700.449320.2±1100349395±20742.3
CE 22:0185.1±43.8181.4±66.6154.1±65.3
CE 22:194.4±3593.7±39.980.5±22.7
CE 22:461.6±29.472±18.972±20.6
CE 22:51013.3±410.81045.9±225.51056.1±331.8
CE 22:614640.6±4365.517865.2±3604.415897.9±6664.7
CE 24:0157.1±66.7166.1±56.8141.1±58.4
CE 24:1151.5±67.3130.3±40.6117.7±30
CE 24:511.2±4.59.9±4.511.9±7.6
CE 24:613.3±13.711.9±4.112.6±8.7
COH919851.6±43495886588.6±126000.8878693.3±260061.4
DG 14:0_14:024.5±29.228±21.780.6±110.5
DG 14:0_16:0458.3±462.2623.8±328.41302±1164.1
DG 14:0_18:11160.6±1314.61325.1±6042139.1±1769.8
DG 14:0_18:2369.9±197.1434.6±274.5666.6±555.3
DG 14:1_16:059.7±93.966.5±41126.9±97.4
DG 16:0_18:17495±5862.410715.9±3041.917638.8±13327.8
DG 16:0_18:22318±916.12589.8±669.24784.2±3174.3
DG 16:0_20:0180.1±113.4174.5±34.5307.6±147.6
DG 16:0_20:3162.1±165.4240.9±99.2401.8±299.6
DG 16:0_20:4388.7±311.5663.2±306.41051.5±898.8
DG 16:0_22:5190.4±45.8281.5±126.8422.3±221.5
DG 16:0_22:6203.2±132.7284±122.7552.8±376.6
DG 16:1_18:13118.2±3175.23348.5±1197.44167.9±2343.6
DG 18:0_16:1217.8±339.7272.2±142.2450.2±423.4
DG 18:0_18:11744.2±1866.32014.4±596.23535.7±3186.2
DG 18:0_18:2824.4±434.3933.8±322.21616.1±1457.3
DG 18:0_20:4252.9±148.4240.2±49.4605.5±397.4
DG 18:1_18:114549.8±9201.713502.3±4060.817117.7±9597.7
DG 18:1_18:210383±4910.37497.6±2434.910707.9±6581.3
DG 18:1_18:31980.3±1524.41303.2±480.42004.9±1623.8
DG 18:1_20:3831.6±581.4681.8±211.2943.7±361.1
DG 18:1_20:42052.9±1339.52009.6±658.12734.6±2134.5
DG 18:2_18:22051.3±1172.51121.7±505.61846.7±1348.7
TG 14:0_16:0_18:22119.9±3113.81681.5±824.21786.2±981
TG 14:0_16:1_18:115689.1±24325.812817±6682.916676±10702.5
TG 14:0_16:1_18:22356.8±2863.11815.6±1350.62527±2157
TG 14:0_18:0_18:1538.5±552.8460.9±131.6462.6±218
TG 14:0_18:2_18:21137.3±518.5713.1±465962.1±755.1
TG 14:1_16:0_18:12913.9±4586.83061.4±1774.13288.7±2061
TG 14:1_16:1_18:011433.7±18825.812185.2±6784.113214.2±11740.7
TG 14:1_18:0_18:2587.9±814367.3±145.7402.8±254.7
TG 14:1_18:1_18:15765.4±5038.34539.6±16354974.2±2103.2
TG 15:0_18:1_16:02349.5±2154.43097±1687.93683.8±2197.4
TG 15:0_18:1_18:1787.1±219.4906.1±477.9802.7±381.6
TG 16:0_16:0_16:02670±2206.54654.4±2248.96093.1±4616.8
TG 16:0_16:0_18:05670.6±3855.17917.6±2955.511025.9±7699.3
TG 16:0_16:0_18:139184.4±32509.956361.6±19969.865983.9±39537.3
TG 16:0_16:0_18:27858.6±3073.810895.8±4724.112934.1±6843.3
TG 16:0_16:1_18:153777.2±54131.958534.3±20253.660182.4±28638.4
TG 16:0_18:0_18:111200.2±12018.914306.1±5315.316854.7±12231.3
TG 16:0_18:1_18:196078.5±49303.2102766.9±29150.694045.1±30306.6
TG 16:0_18:1_18:250245.6±14957.344603.8±14388.145817±19032.6
TG 16:0_18:2_18:210728.3±4558.17949.2±3049.69207.8±4769.3
TG 16:1_16:1_16:11298±1974.5978.3±481.61075.6±613.7
TG 16:1_16:1_18:01118.1±1548.61097.3±591.61628.9±1386.9
TG 16:1_16:1_18:15830.4±6020.46054±2336.55832.5±2611.2
TG 16:1_18:1_18:18334±6440.36584±2595.65665.5±1726.4
TG 16:1_18:1_18:216271.1±8921.311648.1±3692.412053.5±4609.8
TG 17:0_16:0_16:1412.6±119.6578±194.7437.3±165.9
TG 17:0_16:0_18:033.1±29.645.2±24.438.3±15.6
TG 17:0_18:1_14:0256.1±94.5386.3±119.9280.7±154.7
TG 17:0_18:1_16:01904.5±1106.62861.4±1753.52842.9±1672.3
TG 17:0_18:1_16:1282.5±66.4345.2±108.8231.7±71.5
TG 17:0_18:1_18:13862.7±15634070.7±1405.43657.8±1141
TG 17:0_18:2_16:0277.6±60.9310.2±81.5223.6±53.8
TG 18:0_18:0_18:11530.8±2332.71447.7±702.32256.5±2571.5
TG 18:0_18:1_18:113343.8±13301.110862.7±3685.212069.8±8074.6
TG 18:0_18:2_18:23080.7±1786.82469.2±15372553.7±1934.8
TG 18:1_14:0_16:09937.5±11568.411871.3±6719.115809±10286.6
TG 18:1_18:1_18:126949.1±22999.816927±6518.716775.2±8249.9
TG 18:1_18:1_18:210502.4±7340.75232.1±2012.86180.4±4024.4
TG 18:1_18:1_20:41208.6±674.21026.1±2841080.7±620.9
TG 18:1_18:1_22:61655.5±877.71705.9±490.91594.5±831
TG 18:1_18:2_18:211330.7±10454.74571.6±1798.65623±4646.3
TG 18:2_18:2_18:21150.9±1011.2401±172.2558.7±526.4
TG 18:2_18:2_20:4294.9±212.7212.5±88.1285.4±253.6

Abbreviations: dhCer, dihydroceramide; CE, cholesteryl ester; Cer, ceramide; COH, cholesterol; DG, diacylglycerol; DHC, dihexosylceramide; GM3, GM3 ganglioside; MHC, monohexosylceramide; PC, phosphatidylcholine; PC(O), alkylphosphatidylcholine; PC(P), alkenylphosphatidylcholine; PE, phosphatidylethanolamine; PE(O), alkylphosphatidylethanolamine; PE(P), alkenylphosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin; TG, triacylglycerol; THC, trihexosylceramide.

Means ± SD are shown. Concentrations are given in pmol/ml.

p<0.05 vs HIV Negative.

p<0.01 vs HIV Negative.

p<0.05 vs WT HIV.

p<0.01 vs WT HIV (ANOVA).

Experimental design, materials and methods

Patients infected with nef-deficient strain of HIV-1 (ΔNefHIV, n=6) were all members of the SBBC cohort. They were compared with patients infected with Nef-positive (WT) HIV-1 strain (n=6) and six HIV negative subjects selected from a group of healthy volunteers. Description of the patients is presented in the main paper [1]. Lipidomic analysis was performed as described previously [2]. In brief, lipids from 10 μl of plasma were extracted using a modified, single phase Folch method; the analysis was done in triplicates. The analysis was performed by liquid chromatography electrospray ionization-tandem mass spectrometry (LC ESI-MS/MS) using a Agilent 1200 liquid chromatography system, and Applied Biosystems API 4000Q/TRAP mass spectrometer with a turbo-ion spray source (350 °C) and Analyst 1.5 and MultiQuant data systems using a Zorbax C18, 1.8 μm, 50×2.1-mm2 column (Agilent Technologies). Lipid concentrations were calculated by relating the peak area of each species to the peak area of the corresponding internal standard.
Subject areaMedicine
More specific subject areaInfectious diseases/HIV
Type of dataTable
How data was acquiredMass spectroscopy, Agilent 1200 liquid chromatography system, and Applied Biosystems API 4000 Q/TRAP mass spectrometer
Data formatMeans±standard deviations
Experimental factorsPatients infected with wild type and nef-deficient HIV-1 strain
Experimental featuresLipids from 10 μl of plasma were extracted using a modified, single phase Folch method and lipidomic analysis was performed by liquid chromatography electrospray ionization-tandem mass spectrometry
Data source locationMelbourne, Australia
Data accessibilityData is with this article
  2 in total

1.  Lipid metabolism in patients infected with Nef-deficient HIV-1 strain.

Authors:  Hann Low; Lesley Cheng; Maria-Silvana Di Yacovo; Melissa J Churchill; Peter Meikle; Michael Bukrinsky; Andrew F Hill; Dmitri Sviridov
Journal:  Atherosclerosis       Date:  2015-10-30       Impact factor: 5.162

2.  Plasma lipidomic profiling of treated HIV-positive individuals and the implications for cardiovascular risk prediction.

Authors:  Gerard Wong; Janine M Trevillyan; Benoit Fatou; Michelle Cinel; Jacquelyn M Weir; Jennifer F Hoy; Peter J Meikle
Journal:  PLoS One       Date:  2014-04-14       Impact factor: 3.240
  2 in total
  1 in total

1.  Lipid metabolism in patients infected with Nef-deficient HIV-1 strain.

Authors:  Hann Low; Lesley Cheng; Maria-Silvana Di Yacovo; Melissa J Churchill; Peter Meikle; Michael Bukrinsky; Andrew F Hill; Dmitri Sviridov
Journal:  Atherosclerosis       Date:  2015-10-30       Impact factor: 5.162
  1 in total

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