Literature DB >> 26860553

Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy.

Ahmed Ali1, Yasmine Abouleila, Sara Amer, Rie Furushima, Samy Emara, Sebastien Equis, Yann Cotte, Tsutomu Masujima.   

Abstract

The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells' before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.

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Year:  2016        PMID： 26860553     DOI： 10.2116/analsci.32.125

Source DB:  PubMed          Journal:  Anal Sci        ISSN： 0910-6340            Impact factor:   2.081


  10 in total

1.  Metabolomics: Small molecules, single cells.

Authors:  Marissa Fessenden
Journal:  Nature       Date:  2016-11-30       Impact factor: 49.962

2.  [Efficacy and safety of letrozole in treatment of male children with disorders of sex development].

Authors:  Bingqing Yu; Min Nie; Xueyan Wu; Jiangfeng Mao; Xi Wang; Wanlu Ma; Wen Ji; Qibin Huang; Rui Zhang
Journal:  Zhejiang Da Xue Xue Bao Yi Xue Ban       Date:  2020-05-25

3.  Dilute-and-shoot-based direct nano-electrospray ionization tandem mass spectrometry as screening methodology for multivitamins in dietary supplement and human urine.

Authors:  Sara Amer; Walaa Zarad; Heba El-Gendy; Randa Abdel-Salam; Ghada Hadad; Samy Emara; Tsutomu Masujima
Journal:  J Adv Res       Date:  2020-06-12       Impact factor: 10.479

4.  Mass Spectrometry Measurement of Single Suspended Cells Using a Combined Cell Manipulation System and a Single-Probe Device.

Authors:  Shawna J Standke; Devon H Colby; Ryan C Bensen; Anthony W G Burgett; Zhibo Yang
Journal:  Anal Chem       Date:  2019-01-18       Impact factor: 6.986

Review 5.  The role of beta cell heterogeneity in islet function and insulin release.

Authors:  Daniela Nasteska; David J Hodson
Journal:  J Mol Endocrinol       Date:  2018-04-16       Impact factor: 5.098

6.  Endocytosis-mediated mitochondrial transplantation: Transferring normal human astrocytic mitochondria into glioma cells rescues aerobic respiration and enhances radiosensitivity.

Authors:  Chao Sun; Xiongxiong Liu; Bing Wang; Zhenhua Wang; Yang Liu; Cuixia Di; Jing Si; Hongyan Li; Qingfeng Wu; Dan Xu; Ji Li; Gang Li; Yupei Wang; Fang Wang; Hong Zhang
Journal:  Theranostics       Date:  2019-05-26       Impact factor: 11.556

7.  Live single cell mass spectrometry reveals cancer-specific metabolic profiles of circulating tumor cells.

Authors:  Yasmine Abouleila; Kaoru Onidani; Ahmed Ali; Hirokazu Shoji; Takayuki Kawai; Chwee Teck Lim; Vipin Kumar; Shinobu Okaya; Ken Kato; Eiso Hiyama; Toshio Yanagida; Tsutomu Masujima; Yoshihiro Shimizu; Kazufumi Honda
Journal:  Cancer Sci       Date:  2019-01-05       Impact factor: 6.716

8.  Methamphetamine-induced apoptosis in glial cells examined under marker-free imaging modalities.

Authors:  Lianna Y D'Brant; Habben Desta; Ting Chean Khoo; Anna V Sharikova; Supriya D Mahajan; Alexander Khmaladze
Journal:  J Biomed Opt       Date:  2019-04       Impact factor: 3.170

9.  Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux.

Authors:  Patrick A Sandoz; Christopher Tremblay; F Gisou van der Goot; Mathieu Frechin
Journal:  PLoS Biol       Date:  2019-12-19       Impact factor: 8.029

10.  Parvalbumin alters mitochondrial dynamics and affects cell morphology.

Authors:  Lucia Lichvarova; Thomas Henzi; Dzhamilja Safiulina; Allen Kaasik; Beat Schwaller
Journal:  Cell Mol Life Sci       Date:  2018-09-25       Impact factor: 9.261
  10 in total

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