OBJECTIVE: To evaluate the cytogenetic and genotoxic effects of oxalic acid. STUDY DESIGN: Buccal epithelial cells were obtained and cells were suspended in phosphate buffered saline. Increasing amounts of oxalic acids were added to cell suspensions and incubated in 37 degrees C, 5% CO2 for 30 minutes. Comets were scored using a computer-based image analysis system, and tail moments were taken as a measure of DNA damage. The transcriptional changes of HIPK2, GADD45A, DDB2, p53, PCNA, NBS1, and MDM2 genes were also investigated by qRT-PCR using B2M and GAPDH genes as references. RESULTS: It was found that DNA damage occurred in parallel with increasing amounts of oxalic acid according to tail moment measurements, and also expressions of the selected genes have increased. CONCLUSION: It can be concluded that along with increasing amounts of oxalic acid, cell damage was detected, and both comet assay and expression of the selected genes can be used in the assessment of the damage caused by oxalic acid.
OBJECTIVE: To evaluate the cytogenetic and genotoxic effects of oxalic acid. STUDY DESIGN: Buccal epithelial cells were obtained and cells were suspended in phosphate buffered saline. Increasing amounts of oxalic acids were added to cell suspensions and incubated in 37 degrees C, 5% CO2 for 30 minutes. Comets were scored using a computer-based image analysis system, and tail moments were taken as a measure of DNA damage. The transcriptional changes of HIPK2, GADD45A, DDB2, p53, PCNA, NBS1, and MDM2 genes were also investigated by qRT-PCR using B2M and GAPDH genes as references. RESULTS: It was found that DNA damage occurred in parallel with increasing amounts of oxalic acid according to tail moment measurements, and also expressions of the selected genes have increased. CONCLUSION: It can be concluded that along with increasing amounts of oxalic acid, cell damage was detected, and both comet assay and expression of the selected genes can be used in the assessment of the damage caused by oxalic acid.