Mamta Lamba1, Rajni Sharma1, Deepak Sharma2, Mukesh Choudhary3, Rakesh Kumar Maheshwari1. 1. a Department of Microbiology , SMS Medical College , Jaipur , India . 2. b Department of Pediatrics , Pt B.D. Sharma Post Graduate Institute of Medical and Sciences , Rohtak , India , and. 3. c Department of Medical and Paediatric Oncology , GCRI , Ahmedabad , India.
Abstract
BACKGROUND: Neonatal septicaemia is a clinical entity that is characterised by systemic signs and symptoms of infection and accompanied by bacteraemia in first 4 weeks of life and is one of the four leading causes of neonatal mortality and morbidity in India. AIM: To determine the bacterial spectrum and antimicrobial susceptibility pattern of neonatal septicaemia in a tertiary care hospital of North India. MATERIALS AND METHODS: In this prospective observational study, 850 blood samples were collected and processed from clinically suspected neonates according to standard laboratory protocol. Antimicrobial susceptibility of the isolates was done by Kirby Bauer disc diffusion method according to Clinical and Laboratory Standard Institution (CLSI) recommendations. RESULTS: Blood culture reports were positive in 322 (37.8%) cases. Early onset sepsis (EOS) was present in 61.41% and late onset sepsis (LOS) in 38.59% of cases. Gram-negative septicaemia (60.67%) was encountered more than Gram-positive (32.01%). Coagulase negative Staphylococci (17.43%) was the predominant isolate followed by, Klebsiella spp in 16.11% cases. Best overall sensitivity among Gram-negative isolates was to Colistin (89.94%), Imipenem (86.43%) and Meropenam (77.88%). Gram-positive isolates had good (97.15%) sensitivity to linezolid, (95.23%) vancomycin and (88.57%) Teicoplanin. CONCLUSION: Gram-negative organisms are the leading cause of neonatal septicaemia with Klebsiella spp being commonest. Coagulase negative Staphylococci is the predominant isolate among Gram-positive organisms. Most of the isolates are resistant to common antibiotics.
BACKGROUND:Neonatal septicaemia is a clinical entity that is characterised by systemic signs and symptoms of infection and accompanied by bacteraemia in first 4 weeks of life and is one of the four leading causes of neonatal mortality and morbidity in India. AIM: To determine the bacterial spectrum and antimicrobial susceptibility pattern of neonatal septicaemia in a tertiary care hospital of North India. MATERIALS AND METHODS: In this prospective observational study, 850 blood samples were collected and processed from clinically suspected neonates according to standard laboratory protocol. Antimicrobial susceptibility of the isolates was done by Kirby Bauer disc diffusion method according to Clinical and Laboratory Standard Institution (CLSI) recommendations. RESULTS: Blood culture reports were positive in 322 (37.8%) cases. Early onset sepsis (EOS) was present in 61.41% and late onset sepsis (LOS) in 38.59% of cases. Gram-negative septicaemia (60.67%) was encountered more than Gram-positive (32.01%). Coagulase negative Staphylococci (17.43%) was the predominant isolate followed by, Klebsiella spp in 16.11% cases. Best overall sensitivity among Gram-negative isolates was to Colistin (89.94%), Imipenem (86.43%) and Meropenam (77.88%). Gram-positive isolates had good (97.15%) sensitivity to linezolid, (95.23%) vancomycin and (88.57%) Teicoplanin. CONCLUSION: Gram-negative organisms are the leading cause of neonatal septicaemia with Klebsiella spp being commonest. Coagulase negative Staphylococci is the predominant isolate among Gram-positive organisms. Most of the isolates are resistant to common antibiotics.
Authors: Kirsty Sands; Maria J Carvalho; Owen B Spiller; Edward A R Portal; Kathryn Thomson; William John Watkins; Jordan Mathias; Calie Dyer; Chinenye Akpulu; Robert Andrews; Ana Ferreira; Thomas Hender; Rebecca Milton; Maria Nieto; Rabaab Zahra; Haider Shirazi; Adil Muhammad; Shermeen Akif; Muhammad Hilal Jan; Kenneth Iregbu; Fatima Modibbo; Stella Uwaezuoke; Grace J Chan; Delayehu Bekele; Semaria Solomon; Sulagna Basu; Ranjan Kumar Nandy; Sharmi Naha; Jean-Baptiste Mazarati; Aniceth Rucogoza; Lucie Gaju; Shaheen Mehtar; Andre N H Bulabula; Andrew Whitelaw; Timothy R Walsh Journal: BMC Infect Dis Date: 2022-07-01 Impact factor: 3.667
Authors: Raphaël M Zellweger; Buddha Basnyat; Poojan Shrestha; Krishna G Prajapati; Sabina Dongol; Paban K Sharma; Samir Koirala; Thomas C Darton; Christine Boinett; Corinne N Thompson; Guy E Thwaites; Stephen Baker; Abhilasha Karkey Journal: Front Med (Lausanne) Date: 2018-09-19
Authors: Sophie C H Wen; Yukiko Ezure; Lauren Rolley; Geoff Spurling; Colleen L Lau; Saba Riaz; David L Paterson; Adam D Irwin Journal: PLoS Med Date: 2021-09-28 Impact factor: 11.069