Literature DB >> 26855321

TOR (target of rapamycin) is a key regulator of triacylglycerol accumulation in microalgae.

Sousuke Imamura1,2, Yasuko Kawase1, Ikki Kobayashi1, Mie Shimojima3, Hiroyuki Ohta2,3,4, Kan Tanaka1,2.   

Abstract

Most microalgae abundantly accumulate lipid droplets (LDs) containing triacylglycerols (TAGs) under several stress conditions, but the underlying molecular mechanism of this accumulation remains unclear. In a recent study, we found that inhibition of TOR (target of rapamycin), a highly conserved protein kinase of eukaryotes, by rapamycin resulted in TAG accumulation in microalgae, indicating that TOR negatively regulates TAG accumulation. Here, we show that formation of intracellular LDs and TAG accumulation were also induced in the unicellular green alga Chlamydomonas reinhardtii after exposure to Torin1 or AZD8055, which are novel TOR inhibitors that inhibit TOR activity in a manner different from rapamycin. These results supported quite well our previous conclusion that TOR is a central regulator of TAG accumulation in microalgae.

Entities:  

Keywords:  AZD8055; Chlamydomonas reinhardtii; Cyanidioschyzon merolae; TOR inhibitor; Torin1; lipid droplet; microalga; target of rapamycin; triacylglycerol

Mesh：

Substances：

Year:  2016        PMID： 26855321      PMCID： PMC4883934          DOI： 10.1080/15592324.2016.1149285

Source DB:  PubMed          Journal:  Plant Signal Behav        ISSN： 1559-2316


  13 in total

1.  Three acyltransferases and nitrogen-responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas.

Authors:  Nanette R Boyle; Mark Dudley Page; Bensheng Liu; Ian K Blaby; David Casero; Janette Kropat; Shawn J Cokus; Anne Hong-Hermesdorf; Johnathan Shaw; Steven J Karpowicz; Sean D Gallaher; Shannon Johnson; Christoph Benning; Matteo Pellegrini; Arthur Grossman; Sabeeha S Merchant
Journal:  J Biol Chem       Date:  2012-03-08       Impact factor: 5.157

Review 2.  TOR signaling in growth and metabolism.

Authors:  Stephan Wullschleger; Robbie Loewith; Michael N Hall
Journal:  Cell       Date:  2006-02-10       Impact factor: 41.582

Review 3.  Biodiesel from microalgae beats bioethanol.

Authors:  Yusuf Chisti
Journal:  Trends Biotechnol       Date:  2008-01-24       Impact factor: 19.536

Review 4.  Aquatic phototrophs: efficient alternatives to land-based crops for biofuels.

Authors:  G Charles Dismukes; Damian Carrieri; Nicholas Bennette; Gennady M Ananyev; Matthew C Posewitz
Journal:  Curr Opin Biotechnol       Date:  2008-06-06       Impact factor: 9.740

Review 5.  Rapamycin passes the torch: a new generation of mTOR inhibitors.

Authors:  Don Benjamin; Marco Colombi; Christoph Moroni; Michael N Hall
Journal:  Nat Rev Drug Discov       Date:  2011-10-31       Impact factor: 84.694

Review 6.  Biodiesel from microalgae.

Authors:  Yusuf Chisti
Journal:  Biotechnol Adv       Date:  2007-02-13       Impact factor: 14.227

7.  Expression of budding yeast FKBP12 confers rapamycin susceptibility to the unicellular red alga Cyanidioschyzon merolae.

Authors:  Sousuke Imamura; Aiko Ishiwata; Satoru Watanabe; Hirofumi Yoshikawa; Kan Tanaka
Journal:  Biochem Biophys Res Commun       Date:  2013-08-22       Impact factor: 3.575

8.  Glucose-TOR signalling reprograms the transcriptome and activates meristems.

Authors:  Yan Xiong; Matthew McCormack; Lei Li; Qi Hall; Chengbin Xiang; Jen Sheen
Journal:  Nature       Date:  2013-03-31       Impact factor: 49.962

9.  Enhancement of extraplastidic oil synthesis in Chlamydomonas reinhardtii using a type-2 diacylglycerol acyltransferase with a phosphorus starvation-inducible promoter.

Authors:  Masako Iwai; Keiko Ikeda; Mie Shimojima; Hiroyuki Ohta
Journal:  Plant Biotechnol J       Date:  2014-06-09       Impact factor: 9.803

10.  Expression profiling and functional analysis reveals that TOR is a key player in regulating photosynthesis and phytohormone signaling pathways in Arabidopsis.

Authors:  Pan Dong; Fangjie Xiong; Yumei Que; Kai Wang; Lihua Yu; Zhengguo Li; Maozhi Ren
Journal:  Front Plant Sci       Date:  2015-09-07       Impact factor: 5.753
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  15 in total

1.  Overexpression of a glycogenin, CmGLG2, enhances floridean starch accumulation in the red alga Cyanidioschyzon merolae.

Authors:  Imran Pancha; Kan Tanaka; Sousuke Imamura
Journal:  Plant Signal Behav       Date:  2019-04-02

2.  Target of Rapamycin Signaling Involved in the Regulation of Photosynthesis and Cellular Metabolism in Chlorella sorokiniana.

Authors:  Linxuan Li; Tingting Zhu; Lele Huang; Maozhi Ren
Journal:  Int J Mol Sci       Date:  2022-07-04       Impact factor: 6.208

3.  Target of Rapamycin Inhibition in Chlamydomonas reinhardtii Triggers de Novo Amino Acid Synthesis by Enhancing Nitrogen Assimilation.

Authors:  Umarah Mubeen; Jessica Jüppner; Jessica Alpers; Dirk K Hincha; Patrick Giavalisco
Journal:  Plant Cell       Date:  2018-09-18       Impact factor: 11.277

4.  AC-202, a highly effective fluorophore for the visualization of lipid droplets in green algae and diatoms.

Authors:  Seddik Harchouni; Ben Field; Benoît Menand
Journal:  Biotechnol Biofuels       Date:  2018-04-23       Impact factor: 6.040

5.  Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae.

Authors:  Satoshi Fukuda; Eri Hirasawa; Tokiaki Takemura; Sota Takahashi; Kaumeel Chokshi; Imran Pancha; Kan Tanaka; Sousuke Imamura
Journal:  Sci Rep       Date:  2018-08-17       Impact factor: 4.379

Review 6.  The TOR Signaling Network in the Model Unicellular Green Alga Chlamydomonas reinhardtii.

Authors:  María Esther Pérez-Pérez; Inmaculada Couso; José L Crespo
Journal:  Biomolecules       Date:  2017-07-12

7.  Quantitative Phosphoproteomic and System-Level Analysis of TOR Inhibition Unravel Distinct Organellar Acclimation in Chlamydomonas reinhardtii.

Authors:  Valentin Roustan; Wolfram Weckwerth
Journal:  Front Plant Sci       Date:  2018-11-28       Impact factor: 5.753

8.  Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae.

Authors:  Sota Takahashi; Riho Okubo; Yu Kanesaki; Baifeng Zhou; Kazuhiro Takaya; Satoru Watanabe; Kan Tanaka; Sousuke Imamura
Journal:  Plants (Basel)       Date:  2021-05-13

Review 9.  The Unicellular Red Alga Cyanidioschyzon merolae, an Excellent Model Organism for Elucidating Fundamental Molecular Mechanisms and Their Applications in Biofuel Production.

Authors:  Imran Pancha; Kazuhiro Takaya; Kan Tanaka; Sousuke Imamura
Journal:  Plants (Basel)       Date:  2021-06-15

10.  Quantitative in vivo phosphoproteomics reveals reversible signaling processes during nitrogen starvation and recovery in the biofuel model organism Chlamydomonas reinhardtii.

Authors:  Valentin Roustan; Shiva Bakhtiari; Pierre-Jean Roustan; Wolfram Weckwerth
Journal:  Biotechnol Biofuels       Date:  2017-11-28       Impact factor: 6.040
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