PURPOSE: Long non-coding RNAs (lncRNAs) have been shown to play a crucial role in the development as well as the prognosis of various human cancers, particularly in human metastasis associated lung adenocarcinoma transcript-1 (MALAT-1), which has been shown to be upregulated in some tumors. However, the role of MALAT-1 in cervical cancer (CC) is yet to be elucidated. This study aimed to establish a correlation between MALAT-1 and its role in CC progression and prognosis. METHODS: The expression of MALAT-1 was examined in tissue samples of 30 CC patients and was compared with the adjacent non-cancerous tissues. The relationship between MALAT-1 levels and clinicopathological parameters from the CC patients was analyzed. Cell migration and invasion assays were performed with quantification of the expression levels of MALAT-1 in 4 CC cell lines using RT-PCR. RESULTS: Our results demonstrate that MALAT-1 is upregulated in CC. We found higher expression of MALAT-1 in all the 4 CC cell lines, especially in SiHa and ME-180 CC cell lines. After analysis of the relationship between MALAT-1 levels and clinicopathological parameters from CC patients, a robust correlation could be obtained between MALAT-1 overexpression with lymph node metastasis, tumor differentiation and clinical stage. Furthermore, the ectopic expression of MALAT-1 increased cell proliferation and contributed directly to invasion and migration. CONCLUSION: Taken together, this study suggests an indispensible role played by MALAT-1 in CC progression, which may act as a potential prognostic indicator for CC and also could be a novel potential target for diagnosis of CC.
PURPOSE: Long non-coding RNAs (lncRNAs) have been shown to play a crucial role in the development as well as the prognosis of various human cancers, particularly in human metastasis associated lung adenocarcinoma transcript-1 (MALAT-1), which has been shown to be upregulated in some tumors. However, the role of MALAT-1 in cervical cancer (CC) is yet to be elucidated. This study aimed to establish a correlation between MALAT-1 and its role in CC progression and prognosis. METHODS: The expression of MALAT-1 was examined in tissue samples of 30 CC patients and was compared with the adjacent non-cancerous tissues. The relationship between MALAT-1 levels and clinicopathological parameters from the CC patients was analyzed. Cell migration and invasion assays were performed with quantification of the expression levels of MALAT-1 in 4 CC cell lines using RT-PCR. RESULTS: Our results demonstrate that MALAT-1 is upregulated in CC. We found higher expression of MALAT-1 in all the 4 CC cell lines, especially in SiHa and ME-180 CC cell lines. After analysis of the relationship between MALAT-1 levels and clinicopathological parameters from CC patients, a robust correlation could be obtained between MALAT-1 overexpression with lymph node metastasis, tumor differentiation and clinical stage. Furthermore, the ectopic expression of MALAT-1 increased cell proliferation and contributed directly to invasion and migration. CONCLUSION: Taken together, this study suggests an indispensible role played by MALAT-1 in CC progression, which may act as a potential prognostic indicator for CC and also could be a novel potential target for diagnosis of CC.
Authors: Tanvir Alam; Mahmut Uludag; Magbubah Essack; Adil Salhi; Haitham Ashoor; John B Hanks; Craig Kapfer; Katsuhiko Mineta; Takashi Gojobori; Vladimir B Bajic Journal: Nucleic Acids Res Date: 2017-03-17 Impact factor: 16.971
Authors: Stefanie Cerk; Daniela Schwarzenbacher; Jan Basri Adiprasito; Michael Stotz; Georg C Hutterer; Armin Gerger; Hui Ling; George Adrian Calin; Martin Pichler Journal: Int J Mol Sci Date: 2016-09-06 Impact factor: 5.923
Authors: Iulia Virginia Iancu; Gabriela Anton; Anca Botezatu; Irina Huica; Anca Nastase; Demetra Gabriela Socolov; Anca Daniela Stanescu; Simona Olimpia Dima; Nicolae Bacalbasa; Adriana Plesa Journal: J Cell Mol Med Date: 2017-08-02 Impact factor: 5.310