Asmaa M Zahran1, Khalid I Elsayh2, Khaled Saad3, Mostafa Embaby2, Ahmed M Ali2. 1. Clinical Pathology Department, South Egypt Cancer Institute, Assiut University, Egypt. 2. Pediatric Department, Faculty of Medicine, Assiut University, Egypt. 3. Pediatric Department, Faculty of Medicine, Assiut University, Egypt. Electronic address: ksaad8@yahoo.com.
Abstract
BACKGROUND: β-Thalassemia major (BTM) is considered the most common hemoglobinopathy in Egypt and is one of the major health problems in our locality. MATERIALS & METHODS: We investigated the frequency of B-regulatory cells (CD19(+)CD38(hi)CD24(hi)); (Bregs) among polytransfused alloimmunized and non-alloimmunized children with BTM. The study included 110 polytransfused pediatric patients with β-thalassemia major. Clinical and transfusion records of all studied patients were reviewed. Indirect antiglobulin test was performed to detect the presence of alloantibodies. We used flow cytometry for detection of CD19(+)CD38(hi)CD24(hi) regulatory B cells. RESULTS: Alloimmunization was detected in 35.5% of thalassemic patients (39/110). The analysis of our data showed a significantly higher frequency of Bregs (CD19(+)CD38(hi)CD24(hi)) in the peripheral blood of both alloimmunized and non-alloimmunized patients as compared to healthy controls. CONCLUSIONS: Our data showed that the frequencies of CD19(+)CD24(hi)CD38(hi) Bregs cells were significantly increased in children with BTM. Our data suggested that Bregs cells could play a role in the clinical course of BTM. The relationship of Bregs to immune disorders in BTM children remains to be determined. Further longitudinal study with a larger sample size is warranted to explore the mechanisms of Breg cells in the disease process in BTM patients.
BACKGROUND: β-Thalassemia major (BTM) is considered the most common hemoglobinopathy in Egypt and is one of the major health problems in our locality. MATERIALS & METHODS: We investigated the frequency of B-regulatory cells (CD19(+)CD38(hi)CD24(hi)); (Bregs) among polytransfused alloimmunized and non-alloimmunized children with BTM. The study included 110 polytransfused pediatric patients with β-thalassemia major. Clinical and transfusion records of all studied patients were reviewed. Indirect antiglobulin test was performed to detect the presence of alloantibodies. We used flow cytometry for detection of CD19(+)CD38(hi)CD24(hi) regulatory B cells. RESULTS: Alloimmunization was detected in 35.5% of thalassemicpatients (39/110). The analysis of our data showed a significantly higher frequency of Bregs (CD19(+)CD38(hi)CD24(hi)) in the peripheral blood of both alloimmunized and non-alloimmunized patients as compared to healthy controls. CONCLUSIONS: Our data showed that the frequencies of CD19(+)CD24(hi)CD38(hi) Bregs cells were significantly increased in children with BTM. Our data suggested that Bregs cells could play a role in the clinical course of BTM. The relationship of Bregs to immune disorders in BTM children remains to be determined. Further longitudinal study with a larger sample size is warranted to explore the mechanisms of Breg cells in the disease process in BTM patients.
Authors: Asmaa M Zahran; Khalid I Elsayh; Khaled Saad; Mostafa M Embaby; Mervat A M Youssef; Yasser F Abdel-Raheem; Shaban M Sror; Shereen M Galal; Helal F Hetta; Mohamed Diab Aboul-Khair; Mohamd A Alblihed; Amira Elhoufey Journal: Clin Appl Thromb Hemost Date: 2019 Jan-Dec Impact factor: 2.389
Authors: Asmaa M Zahran; Asmaa Nafady; Khaled Saad; Helal F Hetta; Alam-Eldin M Abdallah; Safwat M Abdel-Aziz; Mostafa M Embaby; Amir M Abo Elgheet; Sanaa F Darwish; Mohamed Gamil M Abo-Elela; Amira Elhoufey; Khalid I Elsayh Journal: Clin Appl Thromb Hemost Date: 2020 Jan-Dec Impact factor: 2.389