Justyna Mikuła-Pietrasik1, Krzysztof Książek2. 1. Department of Pathophysiology; Poznań University of Medical Sciences, Poznań, Poland. 2. Department of Pathophysiology; Poznań University of Medical Sciences, Poznań, Poland kksiazek@ump.edu.pl.
Abstract
BACKGROUND/AIM: L-Carnosine inhibits senescence of somatic cells and displays anticancer activity. Here we analyzed if L-carnosine (20 mM) retards senescence of human peritoneal mesothelial cells (HPMCs) and inhibits progression of ovarian cancer cells. MATERIALS AND METHODS: Experiments were performed with primary HPMCs established from patients undergoing abdominal surgery and with three ovarian cancer cell lines: A2780, OVCAR-3 and SKOV-3. RESULTS: L-Carnosine retards senescence of HPMCs plausibly via inhibition of mitochondria-related oxidative stress. Prolonged exposure of HPMCs to L-carnosine prevented senescent HPMC-dependent exacerbation of cancer cell adhesion, migration, invasion and proliferation, which may be linked with decreased secretion of various pro-cancerogenic agents by HPMCs. Cancer cells exposed directly to L-carnosine displayed reduced viability, increased frequency of apoptosis and unaltered proliferation. CONCLUSION: L-carnosine may be a valuable anticancer drug, especially in the context of prevention and therapy of intraperitoneal ovarian cancer metastasis. Copyright
BACKGROUND/AIM: L-Carnosine inhibits senescence of somatic cells and displays anticancer activity. Here we analyzed if L-carnosine (20 mM) retards senescence of human peritoneal mesothelial cells (HPMCs) and inhibits progression of ovarian cancer cells. MATERIALS AND METHODS: Experiments were performed with primary HPMCs established from patients undergoing abdominal surgery and with three ovarian cancer cell lines: A2780, OVCAR-3 and SKOV-3. RESULTS: L-Carnosine retards senescence of HPMCs plausibly via inhibition of mitochondria-related oxidative stress. Prolonged exposure of HPMCs to L-carnosine prevented senescent HPMC-dependent exacerbation of cancer cell adhesion, migration, invasion and proliferation, which may be linked with decreased secretion of various pro-cancerogenic agents by HPMCs. Cancer cells exposed directly to L-carnosine displayed reduced viability, increased frequency of apoptosis and unaltered proliferation. CONCLUSION: L-carnosine may be a valuable anticancer drug, especially in the context of prevention and therapy of intraperitoneal ovarian cancer metastasis. Copyright
Authors: Ivana Jukić; Nikolina Kolobarić; Ana Stupin; Anita Matić; Nataša Kozina; Zrinka Mihaljević; Martina Mihalj; Petar Šušnjara; Marko Stupin; Željka Breškić Ćurić; Kristina Selthofer-Relatić; Aleksandar Kibel; Anamarija Lukinac; Luka Kolar; Gordana Kralik; Zlata Kralik; Aleksandar Széchenyi; Marija Jozanović; Olivera Galović; Martina Medvidović-Kosanović; Ines Drenjančević Journal: Antioxidants (Basel) Date: 2021-06-28