Gabriel Castillo-Dalí1, Raquel Castillo-Oyagüe2, Antonia Terriza3, Jean-Louis Saffar4, Antonio Batista-Cruzado4, Christopher D Lynch5, Alastair J Sloan5, José-Luis Gutiérrez-Pérez1, Daniel Torres-Lagares1. 1. Department of Stomatology, Faculty of Dentistry, University of Seville (US), C/Avicena, s/n, 41009 Seville, Spain. 2. Department of Buccofacial Prostheses, Faculty of Dentistry, Complutense University of Madrid (UCM), Pza. Ramón y Cajal, s/n, 28040 Madrid, Spain. Electronic address: raquel.castillo@ucm.es. 3. Institute of Materials Sciences, Advanced Center of Scientific Research (CSIC), Avda. Américo Vespuccio, no. 49, Isla de la Cartuja, 41092 Seville, Spain. 4. Faculté de Chirurgie Dentaire, Université Paris V- Descartes, rue Maurice Arnoux, no. 1, 92120 Montrouge, Paris, France. 5. School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, CF14 4XY, Cardiff, Wales, UK.
Abstract
OBJECTIVES: Guided bone regeneration (GBR) processes are frequently necessary to achieve appropriate substrates before the restoration of edentulous areas. This study aimed to evaluate the bone regeneration reliability of a new poly-lactic-co-glycolic acid (PLGA) membrane after treatment with oxygen plasma (PO2) and titanium dioxide (TiO2) composite nanoparticles. METHODS: Circumferential bone defects (diameter: 10mm; depth: 3mm) were created on the parietal bones of eight experimentation rabbits and were randomly covered with control membranes (Group 1: PLGA) or experimental membranes (Group 2: PLGA/PO2/TiO2). The animals were euthanized two months afterwards, and a morphologic study was then performed under microscope using ROI (region of interest) colour analysis. Percentage of new bone formation, length of mineralised bone formed in the grown defects, concentration of osteoclasts, and intensity of osteosynthetic activity were assessed. Comparisons among the groups and with the original bone tissue were made using the Kruskal-Wallis test. The level of significance was set in advance at a=0.05. RESULTS: The experimental group recorded higher values for new bone formation, mineralised bone length, and osteoclast concentration; this group also registered the highest osteosynthetic activity. Bone layers in advanced formation stages and low proportions of immature tissue were observed in the study group. CONCLUSIONS: The functionalised membranes showed the best efficacy for bone regeneration. CLINICAL SIGNIFICANCE: The addition of TiO2 nanoparticles onto PLGA/PO2 membranes for GBR processes may be a promising technique to restore bone dimensions and anatomic contours as a prerequisite to well-supported and natural-appearing prosthetic rehabilitations.
OBJECTIVES: Guided bone regeneration (GBR) processes are frequently necessary to achieve appropriate substrates before the restoration of edentulous areas. This study aimed to evaluate the bone regeneration reliability of a new poly-lactic-co-glycolic acid (PLGA) membrane after treatment with oxygen plasma (PO2) and titanium dioxide (TiO2) composite nanoparticles. METHODS: Circumferential bone defects (diameter: 10mm; depth: 3mm) were created on the parietal bones of eight experimentation rabbits and were randomly covered with control membranes (Group 1: PLGA) or experimental membranes (Group 2: PLGA/PO2/TiO2). The animals were euthanized two months afterwards, and a morphologic study was then performed under microscope using ROI (region of interest) colour analysis. Percentage of new bone formation, length of mineralised bone formed in the grown defects, concentration of osteoclasts, and intensity of osteosynthetic activity were assessed. Comparisons among the groups and with the original bone tissue were made using the Kruskal-Wallis test. The level of significance was set in advance at a=0.05. RESULTS: The experimental group recorded higher values for new bone formation, mineralised bone length, and osteoclast concentration; this group also registered the highest osteosynthetic activity. Bone layers in advanced formation stages and low proportions of immature tissue were observed in the study group. CONCLUSIONS: The functionalised membranes showed the best efficacy for bone regeneration. CLINICAL SIGNIFICANCE: The addition of TiO2 nanoparticles onto PLGA/PO2 membranes for GBR processes may be a promising technique to restore bone dimensions and anatomic contours as a prerequisite to well-supported and natural-appearing prosthetic rehabilitations.
Authors: R Velázquez-Cayón; G Castillo-Dalí; J-R Corcuera-Flores; M-A Serrera-Figallo; R Castillo-Oyagüe; M González-Martín; J-L Gutierrez-Pérez; D Torres-Lagares Journal: Med Oral Patol Oral Cir Bucal Date: 2017-09-01
Authors: G Castillo-Dalí; R Castillo-Oyagüe; A Batista-Cruzado; C López-Santos; A Rodríguez-González-Elipe; J-L Saffar; C-D Lynch; J-L Gutiérrez-Pérez; D Torres-Lagares Journal: Med Oral Patol Oral Cir Bucal Date: 2017-03-01