Literature DB >> 26850229

Erratum to: Dynamics of gene silencing during X inactivation using allele-specific RNA-seq.

Hendrik Marks1, Hindrik H D Kerstens2, Tahsin Stefan Barakat3, Erik Splinter4, René A M Dirks2, Guido van Mierlo2, Onkar Joshi2, Shuang-Yin Wang2, Tomas Babak5, Cornelis A Albers6, Tüzer Kalkan7, Austin Smith7, Alice Jouneau8, Wouter de Laat4, Joost Gribnau3, Hendrik G Stunnenberg9.   

Abstract

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Year:  2016        PMID: 26850229      PMCID: PMC4743200          DOI: 10.1186/s13059-016-0885-4

Source DB:  PubMed          Journal:  Genome Biol        ISSN: 1474-7596            Impact factor:   13.583


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After the publication of this work [1], we noticed there was an error in Fig. 5 where −1,0 and 1 are incorrectly displayed in the y-axis in panel b. Please see the corrected Fig. 5 below. We apologize for this error.
Fig. 5

llele-specific RNA-seq on three NPC lines identifies three distal regions of genes that escape XCI. a Ratio of Xi/Xa (y-axis; for each of the three NPC lines sorted from highest to lowest) for genes showing a log2 ratio of at least −5. We set the cutoff for escape on 10 % relative expression from the Xi versus the Xa (log 2 ratio of > −3.32; similar to Yang et al. [37]). b Xi/Xa ratio of genes that escape XCI in all three NPC lines. c Distribution of the escape genes identified in *NPC_129-Xi over the four clusters as characterized in Fig. 4a. d Localization of the escape genes within each NPC line over the linear X chromosome (see also Table 1). The black dots on the fourth row represent all X-linked genes for which high-confidence allele-specific ratios were obtained in NPCs. e Validation of the escape genes within the three escape regions by Sanger sequencing of cDNA. See Additional file 1: Figure S13 for the full panel of 13 genes that we validated, and for further details

llele-specific RNA-seq on three NPC lines identifies three distal regions of genes that escape XCI. a Ratio of Xi/Xa (y-axis; for each of the three NPC lines sorted from highest to lowest) for genes showing a log2 ratio of at least −5. We set the cutoff for escape on 10 % relative expression from the Xi versus the Xa (log 2 ratio of > −3.32; similar to Yang et al. [37]). b Xi/Xa ratio of genes that escape XCI in all three NPC lines. c Distribution of the escape genes identified in *NPC_129-Xi over the four clusters as characterized in Fig. 4a. d Localization of the escape genes within each NPC line over the linear X chromosome (see also Table 1). The black dots on the fourth row represent all X-linked genes for which high-confidence allele-specific ratios were obtained in NPCs. e Validation of the escape genes within the three escape regions by Sanger sequencing of cDNA. See Additional file 1: Figure S13 for the full panel of 13 genes that we validated, and for further details
  1 in total

1.  Dynamics of gene silencing during X inactivation using allele-specific RNA-seq.

Authors:  Hendrik Marks; Hindrik H D Kerstens; Tahsin Stefan Barakat; Erik Splinter; René A M Dirks; Guido van Mierlo; Onkar Joshi; Shuang-Yin Wang; Tomas Babak; Cornelis A Albers; Tüzer Kalkan; Austin Smith; Alice Jouneau; Wouter de Laat; Joost Gribnau; Hendrik G Stunnenberg
Journal:  Genome Biol       Date:  2015-08-03       Impact factor: 13.583

  1 in total
  1 in total

1.  lncRNA-Induced Spread of Polycomb Controlled by Genome Architecture, RNA Abundance, and CpG Island DNA.

Authors:  Megan D Schertzer; Keean C A Braceros; Joshua Starmer; Rachel E Cherney; David M Lee; Gabriela Salazar; Megan Justice; Steven R Bischoff; Dale O Cowley; Pablo Ariel; Mark J Zylka; Jill M Dowen; Terry Magnuson; J Mauro Calabrese
Journal:  Mol Cell       Date:  2019-06-27       Impact factor: 17.970

  1 in total

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