Fawad-Ali Shah1, Sang-Ah Gim2, Jin-Hee Sung2, Seong-Jun Jeon2, Myeong-Ok Kim3, Phil-Ok Koh4. 1. Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea; Division of Life Science and Applied Life Science, Gyeongsang National University, Jinju, South Korea. 2. Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea. 3. Division of Life Science and Applied Life Science, Gyeongsang National University, Jinju, South Korea. 4. Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea. Electronic address: pokoh@gnu.ac.kr.
Abstract
BACKGROUND: Curcumin is known to have a neuroprotective effect against cerebral ischemia. The objective of this study was to identify various proteins that are differentially expressed by curcumin treatment in focal cerebral ischemia using a proteomic approach. METHODS: Adult male rats were treated with vehicle or curcumin 1 h after middle cerebral artery occlusion. Brain tissues were collected 24 h after the onset of middle cerebral artery occlusion, and cerebral cortices proteins were identified by two-dimensional gel electrophoresis and mass spectrometry. RESULTS: We detected several proteins with altered expression levels between vehicle- and curcumin-treated animals. Among these proteins, ubiquitin carboxy-terminal hydrolase L1, isocitrate dehydrogenase, adenosylhomocysteinase, and eukaryotic initiation factor 4A were decreased in the vehicle-treated animal, and curcumin treatment attenuated the injury-induced decreases of these proteins. Conversely, pyridoxal phosphate phosphatase was increased in the vehicle-treated animal, and curcumin treatment prevented decreases in this protein. The identified altered proteins are associated with cellular metabolism and differentiation. CONCLUSIONS: The results of this study suggest that curcumin exerts a neuroprotective effect by regulating the expression of various proteins in focal cerebral ischemia.
BACKGROUND:Curcumin is known to have a neuroprotective effect against cerebral ischemia. The objective of this study was to identify various proteins that are differentially expressed by curcumin treatment in focal cerebral ischemia using a proteomic approach. METHODS: Adult male rats were treated with vehicle or curcumin 1 h after middle cerebral artery occlusion. Brain tissues were collected 24 h after the onset of middle cerebral artery occlusion, and cerebral cortices proteins were identified by two-dimensional gel electrophoresis and mass spectrometry. RESULTS: We detected several proteins with altered expression levels between vehicle- and curcumin-treated animals. Among these proteins, ubiquitin carboxy-terminal hydrolase L1, isocitrate dehydrogenase, adenosylhomocysteinase, and eukaryotic initiation factor 4A were decreased in the vehicle-treated animal, and curcumin treatment attenuated the injury-induced decreases of these proteins. Conversely, pyridoxal phosphate phosphatase was increased in the vehicle-treated animal, and curcumin treatment prevented decreases in this protein. The identified altered proteins are associated with cellular metabolism and differentiation. CONCLUSIONS: The results of this study suggest that curcumin exerts a neuroprotective effect by regulating the expression of various proteins in focal cerebral ischemia.
Authors: Fawad Ali Shah; Gongping Liu; Lina T Al Kury; Alam Zeb; Muzaffar Abbas; Tao Li; Xifei Yang; Fang Liu; Yuhua Jiang; Shupeng Li; Phil-Ok Koh Journal: Front Pharmacol Date: 2019-03-29 Impact factor: 5.810
Authors: Arooj Mohsin Alvi; Lina Tariq Al Kury; Muhammad Umar Ijaz; Fawad Ali Shah; Muhammad Tariq Khan; Ahmed Sadiq Sheikh; Humaira Nadeem; Arif-Ullah Khan; Alam Zeb; Shupeng Li Journal: Biomolecules Date: 2020-05-26
Authors: Fawad Ali Shah; Tao Li; Lina Tariq Al Kury; Alam Zeb; Shehla Khatoon; Gongping Liu; Xifei Yang; Fang Liu; Huo Yao; Arif-Ullah Khan; Phil Ok Koh; Yuhua Jiang; Shupeng Li Journal: Front Neurol Date: 2019-11-14 Impact factor: 4.003