An Martens1, Matteo Montoli2, Giulio Faggi1, Ira Katz3, Jan Pype3, Bart M Vanaudenaerde4, Dirk E M Van Raemdonck2, Arne P Neyrinck5. 1. Laboratory of Anesthesiology and Algology, Department of Cardiovascular Sciences, Katholieke Universiteit Leuven and University Hospitals, Leuven, Belgium; Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven, Belgium. 2. Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven, Belgium; Laboratory of Experimental Thoracic Surgery, Department of Clinical and Experimental Medicine, Katholieke Universiteit Leuven and University Hospitals, Leuven, Belgium. 3. Air Liquide Santé International Medical R&D Paris-Saclay Research Center, Jouy-en Josas, France. 4. Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven, Belgium; Laboratory of Pneumology, Department of Clinical and Experimental Medicine, Lung Transplant Unit, Katholieke Universiteit Leuven and University Hospitals, Leuven, Belgium. 5. Laboratory of Anesthesiology and Algology, Department of Cardiovascular Sciences, Katholieke Universiteit Leuven and University Hospitals, Leuven, Belgium; Leuven Lung Transplant Unit, Katholieke Universiteit Leuven, Leuven, Belgium. Electronic address: arne.neyrinck@uzleuven.be.
Abstract
BACKGROUND: Evidence supports the use of ex vivo lung perfusion (EVLP) as a platform for active reconditioning before transplantation to increase the potential donor pool and to reduce the incidence of primary graft dysfunction. A promising reconditioning strategy is the administration of inhaled noble gases based on their organoprotective effects. Our aim was to validate a porcine warm ischemic lung injury model and investigate postconditioning with argon (Ar) or xenon (Xe) during prolonged EVLP. METHODS: Domestic pigs were divided in four groups (n = 5 per group). In the negative control group, lungs were flushed immediately. In the positive control (PC) and treatment (Ar, Xe) groups, lungs were flushed after a warm ischemic interval of 2-h in situ. All grafts were evaluated and treated during normothermic EVLP for 6 h. In the control groups, lungs were ventilated with 70% N2/30% O2 and in the treatment groups with 70% Ar/30% O2 or 70% Xe/30% O2, respectively. Outcome parameters were physiological variables (pulmonary vascular resistance, peak airway pressures, and PaO2/FiO2), histology, wet-to-dry weight ratio, bronchoalveolar lavage, and computed tomography scan. RESULTS: A significant difference between negative control and PC for pulmonary vascular resistance, peak airway pressures, PaO2/FiO2, wet-to-dry weight ratio, histology, and computed tomography-imaging was observed. No significant differences between the injury group (PC) and the treatment groups (Ar, Xe) were found. CONCLUSIONS: We validated a reproducible prolonged 6-h EVLP model with 2 h of warm ischemia and described the physiological changes over time. In this model, ventilation during EVLP with Ar or Xe administered postinjury did not improve graft function.
BACKGROUND: Evidence supports the use of ex vivo lung perfusion (EVLP) as a platform for active reconditioning before transplantation to increase the potential donor pool and to reduce the incidence of primary graft dysfunction. A promising reconditioning strategy is the administration of inhaled noble gases based on their organoprotective effects. Our aim was to validate a porcine warm ischemic lung injury model and investigate postconditioning with argon (Ar) or xenon (Xe) during prolonged EVLP. METHODS:Domestic pigs were divided in four groups (n = 5 per group). In the negative control group, lungs were flushed immediately. In the positive control (PC) and treatment (Ar, Xe) groups, lungs were flushed after a warm ischemic interval of 2-h in situ. All grafts were evaluated and treated during normothermic EVLP for 6 h. In the control groups, lungs were ventilated with 70% N2/30% O2 and in the treatment groups with 70% Ar/30% O2 or 70% Xe/30% O2, respectively. Outcome parameters were physiological variables (pulmonary vascular resistance, peak airway pressures, and PaO2/FiO2), histology, wet-to-dry weight ratio, bronchoalveolar lavage, and computed tomography scan. RESULTS: A significant difference between negative control and PC for pulmonary vascular resistance, peak airway pressures, PaO2/FiO2, wet-to-dry weight ratio, histology, and computed tomography-imaging was observed. No significant differences between the injury group (PC) and the treatment groups (Ar, Xe) were found. CONCLUSIONS: We validated a reproducible prolonged 6-h EVLP model with 2 h of warm ischemia and described the physiological changes over time. In this model, ventilation during EVLP with Ar or Xe administered postinjury did not improve graft function.
Authors: Shuang Ma; Dongmei Chu; Litao Li; Jennifer A Creed; Yu-Mi Ryang; Huaxin Sheng; Wei Yang; David S Warner; Dennis A Turner; Ulrike Hoffmann Journal: Crit Care Med Date: 2019-08 Impact factor: 7.598
Authors: An Martens; Sofie Ordies; Bart M Vanaudenaerde; Stijn E Verleden; Robin Vos; Geert M Verleden; Eric K Verbeken; Dirk E Van Raemdonck; Sandra Claes; Dominique Schols; Matthieu Chalopin; Ira Katz; Geraldine Farjot; Arne P Neyrinck Journal: Med Gas Res Date: 2017-03-30
Authors: Said Suleiman; Sergej Klassen; Ira Katz; Galina Balakirski; Julia Krabbe; Saskia von Stillfried; Svetlana Kintsler; Till Braunschweig; Aaron Babendreyer; Jan Spillner; Sebastian Kalverkamp; Thomas Schröder; Manfred Moeller; Mark Coburn; Stefan Uhlig; Christian Martin; Annette D Rieg Journal: Sci Rep Date: 2019-02-13 Impact factor: 4.379
Authors: An Martens; Sofie Ordies; Bart M Vanaudenaerde; Stijn E Verleden; Robin Vos; Dirk E Van Raemdonck; Geert M Verleden; Valerie D Roobrouck; Sandra Claes; Dominique Schols; Eric Verbeken; Catherine M Verfaillie; Arne P Neyrinck Journal: Stem Cell Res Ther Date: 2017-07-05 Impact factor: 6.832