OBJECTIVES: Neuro-immune interactions with functional changes in the peripheral blood cells including changes in the transient receptor potential ankyrin 1 (TRPA1) appear to play a pivotal role in the development of chronic pain in humans. The aim of this study was to examine the association between TRPA1 DNA methylation in whole blood cells and the pain states in chronic pain patients. METHODS: After collecting blood samples from 12 chronic pain patients, the authors measured DNA methylation levels in whole blood cells. Significant associations between the patient's demographic data and the chronic pain states were determined by a multiple linear regression analysis that used age, body mass index, pain duration, depression, anxiety, cognitive impairment, activities of daily living, neuropathic pain, and pain states as the dependent variables, and the TRPA1 DNA methylation levels as the independent variables. RESULTS: Multiple regression analysis revealed a significant correlation between increases of the methylation levels of the CpG island in the TRPA1 gene and increases in the number of neuropathic pain symptoms, which were evaluated using the Douleur Neuropathique 4 (DN4) questionnaire. Decreases in the TRPA1 mRNA expression were also significantly related to increases in the DN4 score. The presence of a burning sensation, which is one of pain symptoms in the DN4 questionnaire, was significantly correlated with the increase in DNA methylation level of TRPA1. CONCLUSIONS: TRPA1 DNA methylation levels in whole blood cells appear to be associated with pain symptoms in chronic pain patients.
OBJECTIVES: Neuro-immune interactions with functional changes in the peripheral blood cells including changes in the transient receptor potential ankyrin 1 (TRPA1) appear to play a pivotal role in the development of chronic pain in humans. The aim of this study was to examine the association between TRPA1 DNA methylation in whole blood cells and the pain states in chronic painpatients. METHODS: After collecting blood samples from 12 chronic painpatients, the authors measured DNA methylation levels in whole blood cells. Significant associations between the patient's demographic data and the chronic pain states were determined by a multiple linear regression analysis that used age, body mass index, pain duration, depression, anxiety, cognitive impairment, activities of daily living, neuropathic pain, and pain states as the dependent variables, and the TRPA1 DNA methylation levels as the independent variables. RESULTS: Multiple regression analysis revealed a significant correlation between increases of the methylation levels of the CpG island in the TRPA1 gene and increases in the number of neuropathic pain symptoms, which were evaluated using the Douleur Neuropathique 4 (DN4) questionnaire. Decreases in the TRPA1 mRNA expression were also significantly related to increases in the DN4 score. The presence of a burning sensation, which is one of pain symptoms in the DN4 questionnaire, was significantly correlated with the increase in DNA methylation level of TRPA1. CONCLUSIONS:TRPA1 DNA methylation levels in whole blood cells appear to be associated with pain symptoms in chronic painpatients.
Authors: Jill M Recla; Jason A Bubier; Daniel M Gatti; Jennifer L Ryan; Katie H Long; Raymond F Robledo; Nicole C Glidden; Guoqiang Hou; Gary A Churchill; Richard S Maser; Zhong-Wei Zhang; Erin E Young; Elissa J Chesler; Carol J Bult Journal: Pain Date: 2019-08 Impact factor: 7.926
Authors: Ione Pereira; Saulo J F Mendes; Domingos M S Pereira; Thayanne F Muniz; Valderlane L P Colares; Cinara R A V Monteiro; Mahiba M R de S Martins; Marcos A G Grisotto; Valério Monteiro-Neto; Sílvio G Monteiro; João B Calixto; Susan D Brain; Elizabeth S Fernandes Journal: Front Pharmacol Date: 2017-02-10 Impact factor: 5.810
Authors: Katarzyna Malgorzata Kwiatkowska; Maria Giulia Bacalini; Claudia Sala; Helena Kaziyama; Daniel Ciampi de Andrade; Rossana Terlizzi; Giulia Giannini; Sabina Cevoli; Giulia Pierangeli; Pietro Cortelli; Paolo Garagnani; Chiara Pirazzini Journal: Front Public Health Date: 2020-06-09
Authors: Edwin N Aroke; Paule V Joseph; Abhrarup Roy; Demario S Overstreet; Trygve O Tollefsbol; David E Vance; Burel R Goodin Journal: J Pain Res Date: 2019-02-18 Impact factor: 3.133