Draft Genome Sequence of Bacillus muralis LMG 20238T (DSM 16288), a Spore-Forming Bacterium Isolated from Deteriorated Mural Paintings.

Bacillus muralis LMG 20238(T) is a Gram-positive, aerobic, and spore-forming bacterium. Here, we report the 5.18-Mb draft genome sequence of B. muralis LMG 20238(T), which is the first genome sequence of this species and will promote its fundamental research.

GENOME ANNOUNCEMENT

The type strain LMG 20238T (=DSM 16288T) of Bacillus muralis was isolated from mural paintings in the Lutheran church of Greene-Kreiensen, Germany (1). It is closely related to Bacillus simplex (1). The strains of the B. simplex/B. muralis group can be found frequently in deteriorated wall paintings (1, 2). Except for the taxonomic literature, no additional information of B. muralis has be obtained so far. Because of no available genomic information of B. muralis, its type strain LMG 20238T was selected as one of the research objects in our genome sequencing project for genomic taxonomy and phylogenomics of Bacillus-like bacteria. Here, we present the first draft genome sequence of B. muralis.

The genome sequence of B. muralis LMG 20238T was obtained by paired-end sequencing on the Illumina HiSeq 2500 system. One DNA library with an insert size of 275 bp was constructed and sequenced. After filtering of the 1.18-Gb raw data, the 1.12-Gb clean data were obtained, providing approximately 228-fold coverage. The reads were assembled via SOAPdenovo software version 1.05 (3), using a key parameter K setting at 68. Through the data assembly, 68 scaffolds with a total length of 5,183,886 bp were obtained, and the scaffold N50 was 227,643 bp. The average length of the scaffolds was 76,233 bp, and the longest and shortest scaffolds were 602,085 bp and 539 bp, respectively. A total of 95.12% clean reads could be aligned back to the genome, which covered 98.87% f the sequence.

The annotation of the genome was performed using the NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP) (http://www.ncbi.nlm.nih.gov/books/NBK174280) utilizing GeneMark, Glimmer, and tRNAscan-SE tools (4). A total of 5,392 genes were predicted, including 5,261 coding sequences, 63 tRNAs, and 5 rRNAs. There were 3,624 and 2,268 genes assigned to the COG and KEGG databases, respectively. The average DNA G+C content was 40.95%, agreeing with the value 41.2 mol% (1).

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number LMBV00000000. The version described in this paper is the first version, LMBV01000000.