Draft Genome Sequence of Bacillus plakortidis P203T (DSM 19153), an Alkali- and Salt-Tolerant Marine Bacterium.

Bacillus plakortidis P203(T) is a Gram-positive, spore-forming, and alkali- and salt-tolerant marine bacterium. Here, we report the 3.97-Mb draft genome sequence of B. plakortidis P203(T), which will promote its fundamental research and provide useful information for genomic taxonomy and phylogenomics of Bacillus-like bacteria.

GENOME ANNOUNCEMENT

The type strain P203T (=CIP 107762T) of Bacillus plakortidis was isolated from material from the sponge Plakortis simplex and identified as a novel halo- and alkali-tolerant species of the genus Bacillus (1). Up to now, no additional information of B. plakortidis has be obtained, except that it can be found in an extremely alkaline bauxite residue site of an alumina industrial plant (2). Because of no available genomic information of B. plakortidis, its type strain P203T was selected as one of the research objects in our genome sequencing project for genomic taxonomy and phylogenomics of Bacillus-like bacteria. Here, we present the first draft genome sequence of B. plakortidis.

The genome sequence of B. plakortidis P203T was obtained by paired-end sequencing on the Illumina HiSeq 2500 system. One DNA library with an insert size of 456 bp was constructed and sequenced. After filtering of the 0.55-Gb raw data, the 0.53-Gb clean data were obtained, providing approximately 134-fold coverage. The reads were assembled via SOAPdenovo software version 1.05 (3), using a key parameter K setting at 76. Through the data assembly, 44 scaffolds with a total length of 3,967,762 bp were obtained, and the scaffold N50 was 227,279 bp. The average length of the scaffolds was 90,176 bp, and the longest and shortest scaffolds were 535,841 bp and 673 bp, respectively. A total of 96.36% clean reads could be aligned back to the genome, which covered 98.19% f the sequence.

The annotation of the genome was performed using the NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP) (http://www.ncbi.nlm.nih.gov/genome/annotation_prok) utilizing GeneMark, Glimmer, and tRNAscan-SE tools (4). A total of 4,216 genes were predicted, including 4,143 coding sequences, 68 tRNAs, and 5 rRNAs. There were 2,988 and 1,816 genes assigned to the COG and KEGG databases, respectively. The average DNA G+C content was 39.79 %, agreeing with the value 41.1 mol% (1).

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number LJJD00000000. The version described in this paper is the first version, LJJD01000000.