| Literature DB >> 2684789 |
E Balzi1, W N Chen, E Capieaux, J H McCusker, J E Haber, A Goffeau.
Abstract
In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.Entities:
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Year: 1989 PMID： 2684789 DOI： 10.1016/0378-1119(89)90113-3
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688