Fang Cai1, Hans Hornauer2, Kun Peng3, Cheryl A Schofield1, Heleen Scheerens1, Alyssa M Morimoto1. 1. ITGR/OMNI Biomarker Development, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. 2. Roche Professional Diagnostics, Nonnenwald 2, 82377 Penzberg, Germany. 3. BioAnalytical Sciences, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Abstract
BACKGROUND: IL-13 is a key mediator of type 2 inflammation-driven diseases. Circulating IL-13 levels are very low and challenging to detect reliably. We assessed the ability of immunoassays on the Erenna(®) and IMPACT platforms to measure serum IL-13 in asthma, idiopathic pulmonary fibrosis (IPF) and atopic dermatitis (AD) patients and in healthy controls (HC). RESULTS: The Erenna IL-13 assay exhibited significant specificity issues and had limited ability to detect IL-13 in serum samples. The IMPACT IL-13 assay had excellent specificity and detected IL-13 in 100% of serum samples tested from asthma, IPF and AD patients and HC. Serum IL-13 levels were significantly elevated in asthma, IPF and AD patients, relative to HC. CONCLUSION: The IMPACT IL-13 assay had fg/ml sensitivity and excellent specificity, enabling reliable detection of circulating levels of IL-13.
BACKGROUND:IL-13 is a key mediator of type 2 inflammation-driven diseases. Circulating IL-13 levels are very low and challenging to detect reliably. We assessed the ability of immunoassays on the Erenna(®) and IMPACT platforms to measure serum IL-13 in asthma, idiopathic pulmonary fibrosis (IPF) and atopic dermatitis (AD) patients and in healthy controls (HC). RESULTS: The Erenna IL-13 assay exhibited significant specificity issues and had limited ability to detect IL-13 in serum samples. The IMPACT IL-13 assay had excellent specificity and detected IL-13 in 100% of serum samples tested from asthma, IPF and ADpatients and HC. Serum IL-13 levels were significantly elevated in asthma, IPF and ADpatients, relative to HC. CONCLUSION: The IMPACT IL-13 assay had fg/ml sensitivity and excellent specificity, enabling reliable detection of circulating levels of IL-13.
Authors: Jorge Maspero; Yochai Adir; Mona Al-Ahmad; Carlos A Celis-Preciado; Federico D Colodenco; Pedro Giavina-Bianchi; Hani Lababidi; Olivier Ledanois; Bassam Mahoub; Diahn-Warng Perng; Juan C Vazquez; Arzu Yorgancioglu Journal: ERJ Open Res Date: 2022-08-01