| Literature DB >> 26845352 |
Hiroki Fukuda1, Seikou Nakamura2, Yugo Chisaki3, Tetsuya Takada1, Yuki Toda4, Hiroaki Murata5, Kazuyuki Itoh6, Yoshitaka Yano3, Kazuyuki Takata1, Eishi Ashihara7.
Abstract
Daphnetin, 7,8-dihydroxycoumarin, present in main constituents of Daphne odora var. marginatai, has multiple pharmacological activities including anti-proliferative effects in cancer cells. In this study, using a Transwell system, we showed that daphnetin inhibited invasion and migration of highly metastatic murine osteosarcoma LM8 cells in a dose-dependent manner. Following treatment by daphnetin, cells that penetrated the Transwell membrane were rounder than non-treated cells. Immunofluorescence analysis revealed that daphnetin decreased the numbers of intracellular stress fibers and filopodia. Moreover, daphnetin treatment dramatically decreased the expression levels of RhoA and Cdc42. In summary, the dihydroxycoumarin derivative daphnetin inhibits the invasion and migration of LM8 cells, and therefore represents a promising agent for use against metastatic cancer.Entities:
Keywords: Filopodia; LM8; Metastasis; Rho family small GTP-binding proteins; Stress fiber; daphnetin
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Year: 2016 PMID： 26845352 DOI： 10.1016/j.bbrc.2016.01.179
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575