| Literature DB >> 26843172 |
Patrícia Duarte1,2, Diana Ribeiro1,2,3,4, Inês Carqueijeiro1,2,4, Sara Bettencourt1,2, Mariana Sottomayor5,6,7.
Abstract
The direct uptake of DNA by naked plant cells (protoplasts) provides an expression system of exception for the quickly growing research in non-model plants, fuelled by the power of next-generation sequencing to identify novel candidate genes. Here, we describe a simple and effective method for isolation and transformation of protoplasts, and illustrate its application to several plant materials.Keywords: PEG-mediated transformation; Plant-transferable methodology; Protoplasts; Transient expression
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Year: 2016 PMID： 26843172 DOI： 10.1007/978-1-4939-3393-8_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745