| Literature DB >> 26840893 |
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Abstract
Entities:
Year: 2016 PMID: 26840893 PMCID: PMC4739697 DOI: 10.1371/journal.pone.0148329
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
List of amplified loci indicating their approximate size (bp), annealing temperature (Tm) and the pairs of primers used for the PCR reactions.
| Loci | Size (bp) | Tm (°C) | Primer | Sequence (5'-3') |
|---|---|---|---|---|
| F1 | 3600 | 50 | ORMET [48] | CATAAGCTAATGGGTTCATAC |
| ORRLYS [48] | GAGACCAGTACTTGCTTTCAGTCATC | |||
| F2 | 2100 | 50 | OR16SN | AGAAACCGACCTGGCTCACGC CGG |
| OR12SN | CGTGCCAGCAGCCGCGGTTATACG | |||
| CO1 | 1180 | 58 | SPHCO1F | TAGATCATCAATGGTTAATACAGG |
| SPHCO1R | CTGATATGAGTGTTCTGCAGGAGG | |||
| CO2 | 550 | 58 | C2J3138 [49] | GGAGCTTCACCATTAATAGAACA |
| C2N3661 [49] | CCACAAATTTCTGAACATTGACCA | |||
| 12S | 360 | 58 | SRJ14233 [49] | AAGAGCGACGGGCGATGTGT |
| SRN14588 [49] | AAACTAGGATTAGATACCCTATTAT | |||
| H3 | 329 | 60 | HexAF [50] | ATGGCTCGTACCAAGCAGACGGC |
| HexAR [50] | ATATCCTTGGGCATGATGGTGAC | |||
| ITS2 | 320 | 60 | CAS5p8sFc [51] | TGAACATCGACATTTYGAACGCACAT |
| CAS28sB1d [51] | TTCTTTTCCTCCSCTTAYTRATATGCTTAA |
A source of primers is indicated within brackets and superscript letters.
B designed by H. Song
C designed by S. Sanabria-Urbán
Fig 1Phylogeny based on a concatenated Bayesian analysis of the total genetic evidence retrieved from 67 Sphenarium and 15 outgroup taxa.
Tip labels indicate current taxonomic classification, voucher numbers and locality ID for all included terminals, except for those whose genetic information was retrieved from GenBank (*). Black vertical bars indicate the phylogenetic position of the identified species based on our integrative taxonomy approach (names and coloured symbols in front of the black bars). Particularly, the black vertical bars indicating the phylogenetic position of S. purpurascens, S. variabile, S. macrophallicum, S. sp. nov. 7, S. mexicanum, S. magnum and S. histrio represent cases where species differentiation was primarily morphological and they did not separate in individual monophyletic groups (See methods and results sections for details). The numbers positioned closely to the nodes indicate posterior probability values. G1, Monophyletic Group 1; G2, Monophyletic Group 2; G3, Monophyletic Group 3. S. p. purpurascens (1) intermediate form between S. p. purpurascens and S. p. minimum. S. m. mexicanum (2) intermediate form between S. m. mexicanum and S. m. histrio.