PURPOSE: To describe a new technique for isolation of a mesenchymal stem cells (MSCs) population from the olfactory mucosa in rabbits. METHODS: Olfactory stem cells (OSCs) were retrieved from under the cribriform plate of the Ethmoid bone. Several assays were accomplished to characterize the cell population and attest its viability in vitro. The cells were submitted to flow cytometry with the antibodies CD34, CD45, CD73, CD79, CD90 and CD105 and also they were induced to differentiate in three lineages. Functional evaluation involved analysis of in vitro growth behavior, colony forming unit like fibroblasts (CFU-f) and cryopreservation response. Further transduction with Green Fluorescent Protein (GFP) was also performed. RESULTS: The OSCs showed mesenchymal features, as positive response to CD34, CD73 and CD90 antibodies and plasticity. Additionally, these cells have high proliferated rate, and they could be cultured through many passages and kept the ability to proliferate and differentiate after cryopreservation. The positive response to the transduction signalizes the possibility of cellular tracking in vivo. This is a desirable feature in case those cells are used for pre-clinical trials. CONCLUSION: The cells harvested were mesenchymal stem cells and the technique described is therefore efficient for rabbit olfactory stem cells isolation.
PURPOSE: To describe a new technique for isolation of a mesenchymal stem cells (MSCs) population from the olfactory mucosa in rabbits. METHODS: Olfactory stem cells (OSCs) were retrieved from under the cribriform plate of the Ethmoid bone. Several assays were accomplished to characterize the cell population and attest its viability in vitro. The cells were submitted to flow cytometry with the antibodies CD34, CD45, CD73, CD79, CD90 and CD105 and also they were induced to differentiate in three lineages. Functional evaluation involved analysis of in vitro growth behavior, colony forming unit like fibroblasts (CFU-f) and cryopreservation response. Further transduction with Green Fluorescent Protein (GFP) was also performed. RESULTS: The OSCs showed mesenchymal features, as positive response to CD34, CD73 and CD90 antibodies and plasticity. Additionally, these cells have high proliferated rate, and they could be cultured through many passages and kept the ability to proliferate and differentiate after cryopreservation. The positive response to the transduction signalizes the possibility of cellular tracking in vivo. This is a desirable feature in case those cells are used for pre-clinical trials. CONCLUSION: The cells harvested were mesenchymal stem cells and the technique described is therefore efficient for rabbit olfactory stem cells isolation.
Authors: Rui D Alvites; Mariana V Branquinho; Ana C Sousa; Irina Amorim; Rui Magalhães; Filipa João; Diogo Almeida; Sandra Amado; Justina Prada; Isabel Pires; Federica Zen; Stefania Raimondo; Ana L Luís; Stefano Geuna; Artur S P Varejão; Ana C Maurício Journal: Stem Cells Int Date: 2021-01-02 Impact factor: 5.443
Authors: Antoine D Veron; Cécile Bienboire-Frosini; François Feron; Elisa Codecasa; Arnaud Deveze; Dany Royer; Paul Watelet; Pietro Asproni; Kevin Sadelli; Camille Chabaud; Jean-Claude Stamegna; Joël Fagot; Michel Khrestchatisky; Alessandro Cozzi; François S Roman; Patrick Pageat; Manuel Mengoli; Stéphane D Girard Journal: BMC Vet Res Date: 2018-01-17 Impact factor: 2.741
Authors: Rui D Alvites; Mariana V Branquinho; Ana R Caseiro; Irina Amorim; Sílvia Santos Pedrosa; Alexandra Rêma; Fátima Faria; Beatriz Porto; Cláudia Oliveira; Paula Teixeira; Rui Magalhães; Stefano Geuna; Artur S P Varejão; Ana C Maurício Journal: Int J Cell Biol Date: 2020-01-29