BACKGROUND: Herbal medicine has becoming a potential source of treatment for different types of cancer including breast cancer. It has been shown that plants from the family Rhamnaceae possess anticancer activity. OBJECTIVE: In this study, we determined the antiproliferative influence of Ziziphus spina christi- a species from this family- on the MCF-7 (human breast adenocarcinoma) cell line. MATERIALS AND METHODS: The cytotoxicity of the total extract, ethanol, ethanol-aqueous (1:1) as well as aqueous fractions of Ziziphus spina christi leaves was evaluated through MTT assay against MCF-7 cell line. Cell cycle inhibition and apoptosis induction were assessed by flowcytometry cycle RNase/PI analysis and Annexin V-FLUOS, respectively. Apoptosis was also analyzed by immunoblotting assay. RESULTS: Our results indicated that the ethanolic fraction had the lowest IC50 value (0.02 mg/ml), induced cell cycle arrest at the G1/S phase as well as apoptosis after a 48h of treatment. CONCLUSIONS: This is the first report on anticancer effect of Ziziphus spina christi ethanolic fraction on breast cancer cells, providing a scientific basis for its utility in traditional medicine. However, further in-depth studies are needed to confirm the precise mechanisms.
BACKGROUND:Herbal medicine has becoming a potential source of treatment for different types of cancer including breast cancer. It has been shown that plants from the family Rhamnaceae possess anticancer activity. OBJECTIVE: In this study, we determined the antiproliferative influence of Ziziphus spina christi- a species from this family- on the MCF-7 (humanbreast adenocarcinoma) cell line. MATERIALS AND METHODS: The cytotoxicity of the total extract, ethanol, ethanol-aqueous (1:1) as well as aqueous fractions of Ziziphus spina christi leaves was evaluated through MTT assay against MCF-7 cell line. Cell cycle inhibition and apoptosis induction were assessed by flowcytometry cycle RNase/PI analysis and Annexin V-FLUOS, respectively. Apoptosis was also analyzed by immunoblotting assay. RESULTS: Our results indicated that the ethanolic fraction had the lowest IC50 value (0.02 mg/ml), induced cell cycle arrest at the G1/S phase as well as apoptosis after a 48h of treatment. CONCLUSIONS: This is the first report on anticancer effect of Ziziphus spina christi ethanolic fraction on breast cancer cells, providing a scientific basis for its utility in traditional medicine. However, further in-depth studies are needed to confirm the precise mechanisms.
Authors: Mahmoud Dogara Abdulrahman; Ali Muhammad Zakariya; Harmand A Hama; Saber W Hamad; Sawsan S Al-Rawi; Sarwan W Bradosty; Ahmad H Ibrahim Journal: Adv Pharmacol Pharm Sci Date: 2022-05-29
Authors: Ahmed Ali Abd El-Maksoud; Amal I A Makhlouf; Ammar B Altemimi; Ismail H Abd El-Ghany; Amr Nassrallah; Francesco Cacciola; Tarek Gamal Abedelmaksoud Journal: Molecules Date: 2021-10-21 Impact factor: 4.411