| Literature DB >> 26838168 |
Claudia Gherman1,2, Ovidiu Leonard Braicu2,3,4, Oana Zanoaga2, Anca Jurj2, Valentina Pileczki2, Mahafarin Maralani5, Flaviu Drigla1, Cornelia Braicu2, Liviuta Budisan2, Patriciu Achimas-Cadariu6,7, Ioana Berindan-Neagoe8,9.
Abstract
Ovarian cancer is a highly aggressive pathology, displaying a poor prognosis and chemoresistance to classical therapy. The present study was conducted to evaluate the effect of caffeic acid phenethyl ester (CAPE) on survival of ovarian cancer cell lines, A2780 (sensitive to cisplatin) and A2780cis (resistant to cisplatin). MTT assay was used to evaluate cell viability, while the apoptotic processes were examined by flow cytometry and qRT-PCR. A reduction of cell proliferation and activation of the apoptosis was observed in both cell lines. qRT-PCR evaluation demonstrated the activation of the pro-apoptotic genes (BAD, CASP8, FAS, FADD, p53) in both cell lines. The limited therapeutic effect in A2780 cells is explained by the activation of epithelial-mesenchymal transition-related genes (ZEB1, ZEB2, or TGFBB1) as displayed by Ingenuity Network analysis. Overall data suggest that CAPE can be used as an alternative in sensitizing cells to chemotherapy.Entities:
Keywords: Apoptosis; Caffeic acid phenethyl ester; Epithelial–mesenchymal transition; Ovarian cancer cells
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Year: 2016 PMID： 26838168 DOI： 10.1007/s11010-015-2652-3
Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396