Loribeth Q Evertz1, Sarah M Greising2, Duane A Morrow3, Gary C Sieck2, Kenton R Kaufman3. 1. Mayo Graduate School Biomedical Engineering and Physiology Track, Rochester, Minnesota, USA. 2. Departments of Physiology and Biomedical Engineering, Rochester, Minnesota, USA. 3. Orthopedic Surgery Mayo Clinic, 200 First Street SW, Rochester, Minnesota, 55905, USA.
Abstract
INTRODUCTION: Regional variability in interstitial fluid pressure confounds use of intramuscular pressure measurement to assess muscle force. It is hypothesized that interstitial flow is dependent on intramuscular pressure. The goal of this study was to assess the feasibility of using fluorescent microspheres to evaluate movement of interstitial fluid in skeletal muscle. METHODS: Two diameters of fluorescent microspheres were injected into the rat tibialis anterior during both static (n = 6) and passively lengthened (10% strain) experimental conditions (n = 6). Microsphere dispersion was evaluated using confocal imaging of transverse muscle sections. RESULTS: Fluorescent microspheres tracked interstitial fluid while not penetrating the muscle fiber. When compared with the static condition, significantly greater dispersion (P = 0.003) was seen with passively lengthened conditions (17 ± 9% vs. 31 ± 7%, respectively). Dispersion did not differ for the 2 microsphere sizes (P = 0.811). CONCLUSIONS: Fluorescent microspheres track movement of interstitial fluid, and dispersion is dependent on passive lengthening. Muscle Nerve 54: 444-450, 2016.
INTRODUCTION: Regional variability in interstitial fluid pressure confounds use of intramuscular pressure measurement to assess muscle force. It is hypothesized that interstitial flow is dependent on intramuscular pressure. The goal of this study was to assess the feasibility of using fluorescent microspheres to evaluate movement of interstitial fluid in skeletal muscle. METHODS: Two diameters of fluorescent microspheres were injected into the rat tibialis anterior during both static (n = 6) and passively lengthened (10% strain) experimental conditions (n = 6). Microsphere dispersion was evaluated using confocal imaging of transverse muscle sections. RESULTS: Fluorescent microspheres tracked interstitial fluid while not penetrating the muscle fiber. When compared with the static condition, significantly greater dispersion (P = 0.003) was seen with passively lengthened conditions (17 ± 9% vs. 31 ± 7%, respectively). Dispersion did not differ for the 2 microsphere sizes (P = 0.811). CONCLUSIONS: Fluorescent microspheres track movement of interstitial fluid, and dispersion is dependent on passive lengthening. Muscle Nerve 54: 444-450, 2016.
Authors: Naeem A Ali; James M O'Brien; Stephen P Hoffmann; Gary Phillips; Allan Garland; James C W Finley; Khalid Almoosa; Rana Hejal; Karen M Wolf; Stanley Lemeshow; Alfred F Connors; Clay B Marsh Journal: Am J Respir Crit Care Med Date: 2008-05-29 Impact factor: 21.405
Authors: Taylor M Winters; Genaro S Sepulveda; Patrick S Cottler; Kenton R Kaufman; Richard L Lieber; Samuel R Ward Journal: Muscle Nerve Date: 2009-07 Impact factor: 3.217
Authors: Heleen E Boers; Mohammad Haroon; Fabien Le Grand; Astrid D Bakker; Jenneke Klein-Nulend; Richard T Jaspers Journal: J Orthop Res Date: 2017-12-18 Impact factor: 3.494
Authors: Mohammad Haroon; Jenneke Klein-Nulend; Astrid D Bakker; Jianfeng Jin; Hadi Seddiqi; Carla Offringa; Gerard M J de Wit; Fabien Le Grand; Lorenzo Giordani; Karen J Liu; Robert D Knight; Richard T Jaspers Journal: Biophys J Date: 2021-06-02 Impact factor: 3.699
Authors: Mohammad Haroon; Heleen E Boers; Astrid D Bakker; Niek G C Bloks; Willem M H Hoogaars; Lorenzo Giordani; René J P Musters; Louise Deldicque; Katrien Koppo; Fabien Le Grand; Jenneke Klein-Nulend; Richard T Jaspers Journal: Aging (Albany NY) Date: 2022-01-13 Impact factor: 5.682