G Liu1, W Ji2, J Huang1, L Liu3, Y Wang3. 1. Department of Nephrology, Qingdao Central Hospital, Qingdao, 266042, China. 2. Department of Nephrology, Qingdao Central Hospital, Qingdao, 266042, China. jwxqqd@163.com. 3. Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao, 266071, China.
Abstract
PURPOSE: To investigate 4-HNE expression in diabetic rat kidneys and the protective effect of probucol. METHODS: Diabetic rat models were established. Diabetic rats with successful modeling were randomly divided into the diabetic group (group D) and probucol treated group (group P). Normal rats were put into the control group (group C). Rats in group P were treated with probucol (110 mg/kg day), and rats in groups D and C were given equal volume of water instead. Serum creatinine (SCr), urea nitrogen (BUN), triglyceride (TG), total cholesterol (TC), and 24-h urinary protein were measured at the 4th, 8th, and 12th weeks. Periodic acid-schiff (PAS) staining and hematoxylin-eosin (HE) staining were used to evaluate the renal pathological changes. The immunohistochemistry and Western-blot were used to detect 4-HNE expression in renal tissue. RESULTS: The SCr, BUN, TG, TC, and 24-h urinary protein in group D increased in the 4th, 8th, and 12th weeks and were higher than those in group C (P < 0.05). The SCr, BUN, TG, TC, and 24-h urinary protein in group P decreased compared to group D (P < 0.05). Pathological kidney changes in group D were more serious than those in group P. The level of 4-HNE expression in group D significantly increased at the 4th, 8th, and 12th weeks and were higher than those in group C (P < 0.05). In the kidneys treated with probucol, the level of 4-HNE significantly decreased compared to group D (P < 0.05). CONCLUSIONS: Probucol can protect the diabetic kidney by decreasing 4-HNE expression and lipid peroxidation levels.
PURPOSE: To investigate 4-HNE expression in diabeticrat kidneys and the protective effect of probucol. METHODS:Diabeticrat models were established. Diabeticrats with successful modeling were randomly divided into the diabetic group (group D) and probucol treated group (group P). Normal rats were put into the control group (group C). Rats in group P were treated with probucol (110 mg/kg day), and rats in groups D and C were given equal volume of water instead. Serum creatinine (SCr), ureanitrogen (BUN), triglyceride (TG), total cholesterol (TC), and 24-h urinary protein were measured at the 4th, 8th, and 12th weeks. Periodic acid-schiff (PAS) staining and hematoxylin-eosin (HE) staining were used to evaluate the renal pathological changes. The immunohistochemistry and Western-blot were used to detect 4-HNE expression in renal tissue. RESULTS: The SCr, BUN, TG, TC, and 24-h urinary protein in group D increased in the 4th, 8th, and 12th weeks and were higher than those in group C (P < 0.05). The SCr, BUN, TG, TC, and 24-h urinary protein in group P decreased compared to group D (P < 0.05). Pathological kidney changes in group D were more serious than those in group P. The level of 4-HNE expression in group D significantly increased at the 4th, 8th, and 12th weeks and were higher than those in group C (P < 0.05). In the kidneys treated with probucol, the level of 4-HNE significantly decreased compared to group D (P < 0.05). CONCLUSIONS:Probucol can protect the diabetic kidney by decreasing 4-HNE expression and lipid peroxidation levels.
Authors: Kristofer S Fritz; James J Galligan; Rebecca L Smathers; James R Roede; Colin T Shearn; Philip Reigan; Dennis R Petersen Journal: Chem Res Toxicol Date: 2011-04-14 Impact factor: 3.739
Authors: A Sundqvist; A Zieba; E Vasilaki; C Herrera Hidalgo; O Söderberg; D Koinuma; K Miyazono; C-H Heldin; U Landegren; P Ten Dijke; H van Dam Journal: Oncogene Date: 2012-08-27 Impact factor: 9.867
Authors: David Philipp Wohlfart; Bowen Lou; Chiara Simone Middel; Jakob Morgenstern; Thomas Fleming; Carsten Sticht; Ingrid Hausser; Rüdiger Hell; Hans-Peter Hammes; Julia Szendrödi; Peter Paul Nawroth; Jens Kroll Journal: Redox Biol Date: 2022-01-26 Impact factor: 11.799
Authors: Bowen Lou; Mike Boger; Katrin Bennewitz; Carsten Sticht; Stefan Kopf; Jakob Morgenstern; Thomas Fleming; Rüdiger Hell; Zuyi Yuan; Peter Paul Nawroth; Jens Kroll Journal: Redox Biol Date: 2020-09-16 Impact factor: 11.799