A calixpyridinium-based supramolecular tandem assay for alkaline phosphatase and its application to ATP hydrolysis reaction.

We have successfully implemented the supramolecular tandem assay principle for the real-time, continuous, direct, and label-free monitoring of alkaline phosphatase activity through a fluorescence "switch-off" assay based on a novel calixpyridinium/dye reporter pair. Because several diseases can be preliminarily diagnosed in light of an abnormal level of alkaline phosphatase in serum, the application of tandem assays to selectively monitor alkaline phosphatase activity has feasible implications in disease diagnosis.