Celia Carrillo1, María Giraldo2, M Mar Cavia2, Sara R Alonso-Torre2. 1. Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain. ccarrillo@ubu.es. 2. Nutrition and Food Science, Faculty of Sciences, University of Burgos, Pl. Misael Bañuelos s/n, 09001, Burgos, Spain.
Abstract
PURPOSE: To study the mechanism by which oleic acid (OA) (C18:1) exerts its beneficial effects on immune-competent cells. Since store-operated Ca2+ entry (SOCE) is a Ca2+ influx pathway involved in the control of multiple physiological processes including cell proliferation, we studied the effect of OA in Ca2+ signals of Jurkat T cells and THP-1 monocytes, paying particular attention to SOCE. METHODS: Changes in [Ca2+]i were measured using the Fura-2 fluorescence dye. Mn2+ uptake was monitored as a rate of quenching of Fura-2 fluorescence measured at the Ca2+-insensitive wavelengths. Thapsigargin was used to induce SOCE in Fura-2-loaded cells. RESULTS: We showed a clear dose-dependent SOCE-inhibitory effect of OA in both cell lines. Such an inhibitory effect was PKC independent and totally restored by albumin, suggesting that OA exerts its effect somewhere in the membrane. We also demonstrated that OA induces increases in [Ca2+]i partly mediated by an extracellular Ca2+ influx through econazole-insensitive channels. Finally, we compared the effect of OA with stearic acid (C18:0), assuming the emerged evidence concerning the link between saturated fats and inflammation disorders. Stearic acid failed to inhibit SOCE, independently on the concentration tested, thus intensifying the physiological relevance of our findings. CONCLUSION: We suggest a physiological pathway for the beneficial effects of OA in inflammation.
PURPOSE: To study the mechanism by which oleic acid (OA) (C18:1) exerts its beneficial effects on immune-competent cells. Since store-operated Ca2+ entry (SOCE) is a Ca2+ influx pathway involved in the control of multiple physiological processes including cell proliferation, we studied the effect of OA in Ca2+ signals of Jurkat T cells and THP-1 monocytes, paying particular attention to SOCE. METHODS: Changes in [Ca2+]i were measured using the Fura-2 fluorescence dye. Mn2+ uptake was monitored as a rate of quenching of Fura-2 fluorescence measured at the Ca2+-insensitive wavelengths. Thapsigargin was used to induce SOCE in Fura-2-loaded cells. RESULTS: We showed a clear dose-dependent SOCE-inhibitory effect of OA in both cell lines. Such an inhibitory effect was PKC independent and totally restored by albumin, suggesting that OA exerts its effect somewhere in the membrane. We also demonstrated that OA induces increases in [Ca2+]i partly mediated by an extracellular Ca2+ influx through econazole-insensitive channels. Finally, we compared the effect of OA with stearic acid (C18:0), assuming the emerged evidence concerning the link between saturated fats and inflammation disorders. Stearic acid failed to inhibit SOCE, independently on the concentration tested, thus intensifying the physiological relevance of our findings. CONCLUSION: We suggest a physiological pathway for the beneficial effects of OA in inflammation.
Authors: Celia Carrillo; Aziz Hichami; Pierre Andreoletti; Mustapha Cherkaoui-Malki; María del Mar Cavia; Souleymane Abdoul-Azize; Sara R Alonso-Torre; Naim Akhtar Khan Journal: Biochim Biophys Acta Date: 2012-01-25
Authors: Helioswilton Sales-Campos; Patricía Reis de Souza; Bethânea Crema Peghini; João Santana da Silva; Cristina Ribeiro Cardoso Journal: Mini Rev Med Chem Date: 2013-02 Impact factor: 3.862
Authors: Jaqueline Mena; Carolina Manosalva; Ruben Ramirez; Lhia Chandia; Daniel Carroza; Anitsi Loaiza; Rafael A Burgos; Maria A Hidalgo Journal: Vet Immunol Immunopathol Date: 2012-12-05 Impact factor: 2.046