| Literature DB >> 26830053 |
Zofia Bakuła1, Magdalena Modrzejewska2, Aleksandra Safianowska3, Jakko van Ingen4, Małgorzata Proboszcz5, Jacek Bielecki6, Tomasz Jagielski7.
Abstract
Within this study, a new, rapid method for subtyping of Mycobacterium kansasii was developed based on the sequence analysis of the tuf gene coding for the Tu (thermo-unstable) elongation factor (EF-Tu). The method involves PCR amplification of ca. 740-bp tuf gene fragment, followed by digestion with the MvaI restriction endonuclease.Entities:
Keywords: Mycobacterium kansasii; PCR restriction enzyme analysis; Subtyping; tuf
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Year: 2015 PMID: 26830053 DOI: 10.1016/j.diagmicrobio.2015.12.009
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN: 0732-8893 Impact factor: 2.803