Aina Teniente-Serra1,2, Laia Grau-López3, M José Mansilla1,2, Marco Fernández-Sanmartín4, Anna Ester Condins5, Cristina Ramo-Tello3, Eva Martínez-Cáceres1,2. 1. a Immunology Division, Germans Trias i Pujol University Hospital and Research Institute (IGTP) , Campus Can Ruti , Badalona , Barcelona . 2. b Department of Cell Biology , Physiology and Immunology, Universitat Autònoma de Barcelona , Bellaterra , Barcelona . 3. c Multiple Sclerosis Unit, Department of Neurosciences. Germans Trias i Pujol University Hospital , Badalona , Barcelona , and. 4. d Flow Cytometry Facility, Germans Trias i Pujol Research Institute (IGTP) , Campus Can Ruti , Badalona , Barcelona , and. 5. e Blood and Tissue Bank (BST) , Barcelona , Spain.
Abstract
OBJECTIVE: The objective of this study is to characterise the functionally relevant minor lymphocyte subpopulations in whole blood of multiple sclerosis (MS) patients and their potential utility as biomarkers for treatment follow up. MATERIAL AND METHODS: Peripheral blood from 40 healthy donors (HD) and 66 MS patients [23 relapsing-remitting (RRMS) without treatment, 27 RRMS undergoing treatment (16 IFN-β, 11 natalizumab), and 16 progressive forms (eight secondary progressive and eight primary progressive)] was analysed by multiparametric flow cytometry. RESULTS: Untreated MS patients showed a decrease in early effector memory (CD45RA(-)CCR7(-)CD27(+)) CD4(+) and CD8(+) T cells and an increase in Th17 lymphocytes in peripheral blood compared with HD. Regarding the effect of treatment, whereas no differences in relative percentages of cellular subpopulations were observed in patients under IFN-β treatment, those under treatment with natalizumab had an increased percentage of early effector memory CD4(+) (CD45RA(-)CCR7(-)CD27(+)), central memory CD8(+) (CD45RA(-)CCR7(+)CD27(+)) T cells, recent thymic emigrants (CD4(+) CD45RA(+)CCR7(+)CD27(+)CD31(+)PTK7(+)) and transitional B cells (CD19(+)CD27(-)CD24(hi)CD38(hi)). CONCLUSIONS: Multiparametric flow cytometry analysis of whole blood is a robust, reproducible, and sensitive technology to monitor the effect of MS treatments even in minor lymphocyte subpopulations that might represent useful biomarkers of treatment response.
OBJECTIVE: The objective of this study is to characterise the functionally relevant minor lymphocyte subpopulations in whole blood of multiple sclerosis (MS) patients and their potential utility as biomarkers for treatment follow up. MATERIAL AND METHODS: Peripheral blood from 40 healthy donors (HD) and 66 MSpatients [23 relapsing-remitting (RRMS) without treatment, 27 RRMS undergoing treatment (16 IFN-β, 11 natalizumab), and 16 progressive forms (eight secondary progressive and eight primary progressive)] was analysed by multiparametric flow cytometry. RESULTS: Untreated MSpatients showed a decrease in early effector memory (CD45RA(-)CCR7(-)CD27(+)) CD4(+) and CD8(+) T cells and an increase in Th17 lymphocytes in peripheral blood compared with HD. Regarding the effect of treatment, whereas no differences in relative percentages of cellular subpopulations were observed in patients under IFN-β treatment, those under treatment with natalizumab had an increased percentage of early effector memory CD4(+) (CD45RA(-)CCR7(-)CD27(+)), central memory CD8(+) (CD45RA(-)CCR7(+)CD27(+)) T cells, recent thymic emigrants (CD4(+) CD45RA(+)CCR7(+)CD27(+)CD31(+)PTK7(+)) and transitional B cells (CD19(+)CD27(-)CD24(hi)CD38(hi)). CONCLUSIONS: Multiparametric flow cytometry analysis of whole blood is a robust, reproducible, and sensitive technology to monitor the effect of MS treatments even in minor lymphocyte subpopulations that might represent useful biomarkers of treatment response.
Authors: Bibiana Quirant-Sánchez; José V Hervás-García; Aina Teniente-Serra; Luis Brieva; Ester Moral-Torres; Antonio Cano; Elvira Munteis; María J Mansilla; Silvia Presas-Rodriguez; Juan Navarro-Barriuso; Cristina Ramo-Tello; Eva M Martínez-Cáceres Journal: CNS Neurosci Ther Date: 2018-04-15 Impact factor: 5.243
Authors: Sonja Dulic; Zsófia Vásárhelyi; Florentina Sava; László Berta; Balázs Szalay; Gergely Toldi; László Kovács; Attila Balog Journal: Mediators Inflamm Date: 2017-10-25 Impact factor: 4.711
Authors: G Cull; D Hall; M J Fabis-Pedrini; W M Carroll; L Forster; F Robins; R Ghassemifar; C Crosbie; S Walters; I James; B Augustson; A K Kermode Journal: Mult Scler J Exp Transl Clin Date: 2017-03-23
Authors: Emanuele D'Amico; Aurora Zanghì; Nunziatina Laura Parrinello; Alessandra Romano; Giuseppe Alberto Palumbo; Clara Grazia Chisari; Simona Toscano; Francesco Di Raimondo; Mario Zappia; Francesco Patti Journal: Front Immunol Date: 2022-02-22 Impact factor: 7.561