Sonja Jacobsen1, Pranav Patel1, Jonas Schmidt-Chanasit2, Isabelle Leparc-Goffart3, Anette Teichmann1, Herve Zeller4, Matthias Niedrig5. 1. Centre for Biological Threats and Special Pathogens, Highly Pathogenic Viruses, Robert Koch Institute, Berlin, Germany. 2. WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany. 3. Centre national de référence des arbovirus, Errit-IRBA HIA Laveran, Marseille, France. 4. European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden. 5. Centre for Biological Threats and Special Pathogens, Highly Pathogenic Viruses, Robert Koch Institute, Berlin, Germany. Electronic address: niedrigm@rki.de.
Abstract
BACKGROUND: Since the re-emergence of Chikungunya virus (CHIKV) in Reunion in 2005 and the recent outbreak in the Caribbean islands with an expansion to the Americas the CHIK diagnostic became very important. OBJECTIVES: We evaluate the performance of laboratories regarding molecular and serological diagnostic of CHIK worldwide. STUDY DESIGN: A panel of 12 samples for molecular and 13 samples for serology were provided to 60 laboratories in 40 countries for evaluating the sensitivity and specificity of molecular and serology testing. RESULTS: The panel for molecular diagnostic testing was analysed by 56 laboratories returning 60 data sets of results whereas the 56 and 60 data sets were returned for IgG and IgM diagnostic from the participating laboratories. Twenty-three from 60 data sets performed optimal, 7 acceptable and 30 sets of results require improvement. From 50 data sets only one laboratory shows an optimal performance for IgM detection, followed by 9 data sets with acceptable and the rest need for improvement. From 46 IgG serology data sets 20 provide an optimal, 2 an acceptable and 24 require improvement performance. The evaluation of some of the diagnostic performances allows linking the quality of results to the in-house methods or commercial assays used. CONCLUSION: The external quality assurance for CHIK diagnostics provides a good overview on the laboratory performance regarding sensitivity and specificity for the molecular and serology diagnostic required for the quick and reliable analysis of suspected CHIK patients. Nearly half of the laboratories have to improve their diagnostic profile to achieve a better performance.
BACKGROUND: Since the re-emergence of Chikungunya virus (CHIKV) in Reunion in 2005 and the recent outbreak in the Caribbean islands with an expansion to the Americas the CHIK diagnostic became very important. OBJECTIVES: We evaluate the performance of laboratories regarding molecular and serological diagnostic of CHIK worldwide. STUDY DESIGN: A panel of 12 samples for molecular and 13 samples for serology were provided to 60 laboratories in 40 countries for evaluating the sensitivity and specificity of molecular and serology testing. RESULTS: The panel for molecular diagnostic testing was analysed by 56 laboratories returning 60 data sets of results whereas the 56 and 60 data sets were returned for IgG and IgM diagnostic from the participating laboratories. Twenty-three from 60 data sets performed optimal, 7 acceptable and 30 sets of results require improvement. From 50 data sets only one laboratory shows an optimal performance for IgM detection, followed by 9 data sets with acceptable and the rest need for improvement. From 46 IgG serology data sets 20 provide an optimal, 2 an acceptable and 24 require improvement performance. The evaluation of some of the diagnostic performances allows linking the quality of results to the in-house methods or commercial assays used. CONCLUSION: The external quality assurance for CHIK diagnostics provides a good overview on the laboratory performance regarding sensitivity and specificity for the molecular and serology diagnostic required for the quick and reliable analysis of suspected CHIK patients. Nearly half of the laboratories have to improve their diagnostic profile to achieve a better performance.
Authors: Li Ting Soh; Raynal C Squires; Li Kiang Tan; Kwoon Yong Pok; HuiTing Yang; Christina Liew; Aparna Singh Shah; John Aaskov; Sazaly Abubakar; Futoshi Hasabe; Lee Ching Ng; Frank Konings Journal: Western Pac Surveill Response J Date: 2016-04-22
Authors: Giulietta Venturi; Stephan W Aberle; Tatjana Avšič-Županc; Luisa Barzon; Christoph Batejat; Elisa Burdino; Fabrizio Carletti; Rémi Charrel; Iva Christova; Jeff Connell; Victor Max Corman; Mary Emmanouil; Anne J Jääskeläinen; Ivan Kurolt; Yaniv Lustig; Miguel J Martinez; Marion Koopmans; Orsolya Nagy; Trung Nguyen; Anna Papa; Mercedes Pérez-Ruiz; Martin Pfeffer; Jelena Protic; Johan Reimerink; Giada Rossini; María Paz Sánchez-Seco Fariñas; Jonas Schmidt-Chanasit; Sandra Söderholm; Bertrand Sudre; Marjan Van Esbroeck; Chantal B Reusken Journal: Euro Surveill Date: 2020-04