Panagiota Tyrnenopoulou1, Nikolaos Diakakis1, Maria Karayannopoulou2, Ioannis Savvas3, Georgios Koliakos4. 1. a Equine Unit, Companion Animal Clinic, Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece. 2. b Surgery and Obstetrics Unit, Companion Animal Clinic, Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece. 3. c Anaesthesiology and Intensive Care Unit, Companion Animal Clinic, Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece. 4. d Department of Biochemistry, Medical School , Faculty of Health Sciences , Aristotle University of Thessaloniki , Thessaloniki , Greece.
Abstract
BACKGROUND: Regenerative medicine has become one of the most promising therapies of equine osteoarthritis. Platelet lysate (PL) is rich in bioactive proteins and growth factors that play a crucial role in tissue healing. OBJECTIVE: To evaluate the efficacy of intra-articularly injected autologous PL in equine athletes with naturally occurring osteoarthritis. ANIMALS AND METHODS: Fifteen warmblood geldings aged 8-19 years with osteoarthritis of the distal interphalangeal joint were included in this study. They were randomly divided into two groups; 10 horses received intra-articular injections of PL and 5 of normal saline (controls). Before treatment, platelet-derived growth factor (PDGF) levels in basal plasma and prepared PL were estimated. Each joint was injected twice within a three-week period. Lameness was evaluated using the American Association of Equine Practitioners grading system, before treatment and 10 days after each intra-articular injection. Horses were examined fortnightly for one year. Radiographic examination was performed six months post-treatment. The generalized estimating equation test was used for statistical analysis. RESULTS: Acceptable levels of PDGF were detected in PLs (mean ± SD: 258.0 ± 52.3 pg/ml). The majority of horses (9/10) responded positively to PL treatment presenting lower lameness grades (p < 0.0005) compared to controls 10 days after the second injection, and returned to normal athletic activity. Radiographs revealed no changes in osteoarthritis lesions six months after treatment. One year post-injections, however, all horses relapsed to their initial degree of lameness. CONCLUSION: Intra-articularly injected autologous PL is an efficient method for temporarily managing osteoarthritis of the distal interphalangeal joint in athletic horses.
BACKGROUND: Regenerative medicine has become one of the most promising therapies of equineosteoarthritis. Platelet lysate (PL) is rich in bioactive proteins and growth factors that play a crucial role in tissue healing. OBJECTIVE: To evaluate the efficacy of intra-articularly injected autologous PL in equine athletes with naturally occurring osteoarthritis. ANIMALS AND METHODS: Fifteen warmblood geldings aged 8-19 years with osteoarthritis of the distal interphalangeal joint were included in this study. They were randomly divided into two groups; 10 horses received intra-articular injections of PL and 5 of normal saline (controls). Before treatment, platelet-derived growth factor (PDGF) levels in basal plasma and prepared PL were estimated. Each joint was injected twice within a three-week period. Lameness was evaluated using the American Association of Equine Practitioners grading system, before treatment and 10 days after each intra-articular injection. Horses were examined fortnightly for one year. Radiographic examination was performed six months post-treatment. The generalized estimating equation test was used for statistical analysis. RESULTS: Acceptable levels of PDGF were detected in PLs (mean ± SD: 258.0 ± 52.3 pg/ml). The majority of horses (9/10) responded positively to PL treatment presenting lower lameness grades (p < 0.0005) compared to controls 10 days after the second injection, and returned to normal athletic activity. Radiographs revealed no changes in osteoarthritis lesions six months after treatment. One year post-injections, however, all horses relapsed to their initial degree of lameness. CONCLUSION: Intra-articularly injected autologous PL is an efficient method for temporarily managing osteoarthritis of the distal interphalangeal joint in athletic horses.
Authors: Jessica M Gilbertie; Thomas P Schaer; Alicia G Schubert; Megan E Jacob; Stefano Menegatti; R Ashton Lavoie; Lauren V Schnabel Journal: J Orthop Res Date: 2020-01-14 Impact factor: 3.494
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