| Literature DB >> 26827770 |
Fernando Campanhã Vicentini1, Lívia L C Garcia2, Luiz C S Figueiredo-Filho3, Bruno C Janegitz4, Orlando Fatibello-Filho2.
Abstract
In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5×10(-6) to 9.5×10(-5)molL(-1) with a detection limit of 1.7×10(-7)molL(-1). The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method.Entities:
Keywords: Amperometry.; Catechol determination; Dihexadecylphosphate; Gold nanoparticles; Tyrosinase biosensor
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Year: 2015 PMID： 26827770 DOI： 10.1016/j.enzmictec.2015.12.004
Source DB: PubMed Journal: Enzyme Microb Technol ISSN： 0141-0229 Impact factor: 3.493