| Literature DB >> 26826385 |
Suvasmita Rath1, Aditya Anand1, Nilabh Ghosh1, Lopamudra Das1, Shrikant B Kokate1, Pragyesh Dixit1, Swetapadma Majhi1, Niranjan Rout2, Shivaram P Singh3, Asima Bhattacharyya4.
Abstract
Hypoxia promotes cancer progression, and metastasis. The major protein expressed in hypoxic solid cancer is hypoxia-inducible factor 1 (HIF1). We show that enhanced phosphorylation of a conventional protein kinase C isoform, PKCα, at threonine 638 (T(638)) by hypoxia-mimetic cobalt chloride induces HIF1α in nuclei of gastric epithelial cells (GECs). Moreover, phospho-T(638)-PKCα (P-PKCα) interacts with p300-HIF1α complex in the nuclei of hypoxic GECs and PKCα phosphorylation at T(638) enhances transcriptional activity of HIF1α. High P-PKCα expression in neoplastic gastric cancer biopsy samples as compared to nonneoplastic samples suggests that P-PKCα might act as an indicator of gastric cancer progression.Entities:
Keywords: Cobalt chloride; Gastric epithelial cell; HIF1α; Hypoxia; PKC; p300
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Year: 2016 PMID： 26826385 DOI： 10.1016/j.bbrc.2016.01.140
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575