Literature DB >> 26820064

Cardiovascular malformations caused by NOTCH1 mutations do not keep left: data on 428 probands with left-sided CHD and their families.

Wilhelmina S Kerstjens-Frederikse1, Ingrid M B H van de Laar2, Yvonne J Vos1, Judith M A Verhagen2, Rolf M F Berger3, Klaske D Lichtenbelt4, Jolien S Klein Wassink-Ruiter1, Paul A van der Zwaag1, Gideon J du Marchie Sarvaas3, Klasien A Bergman5, Catia M Bilardo6, Jolien W Roos-Hesselink7, Johan H P Janssen8, Ingrid M Frohn-Mulder9, Karin Y van Spaendonck-Zwarts1, Joost P van Melle3, Robert M W Hofstra1,2,10, M W Wessels2.   

Abstract

PURPOSE: We aimed to determine the prevalence and phenotypic spectrum of NOTCH1 mutations in left-sided congenital heart disease (LS-CHD). LS-CHD includes aortic valve stenosis, a bicuspid aortic valve, coarctation of the aorta, and hypoplastic left heart syndrome.
METHODS: NOTCH1 was screened for mutations in 428 nonsyndromic probands with LS-CHD, and family histories were obtained for all. When a mutation was detected, relatives were also tested.
RESULTS: In 148/428 patients (35%), LS-CHD was familial. Fourteen mutations (3%; 5 RNA splicing mutations, 8 truncating mutations, 1 whole-gene deletion) were detected, 11 in familial disease (11/148 (7%)) and 3 in sporadic disease (3/280 (1%)). Forty-nine additional mutation carriers were identified among the 14 families, of whom 12 (25%) were asymptomatic. Most of these mutation carriers had LS-CHD, but 9 (18%) had right-sided congenital heart disease (RS-CHD) or conotruncal heart disease (CTD). Thoracic aortic aneurysms (TAAs) occurred in 6 mutation carriers (probands included 6/63 (10%)).
CONCLUSION: Pathogenic mutations in NOTCH1 were identified in 7% of familial LS-CHD and in 1% of sporadic LS-CHD. The penetrance is high; a cardiovascular malformation was found in 75% of NOTCH1 mutation carriers. The phenotypic spectrum includes LS-CHD, RS-CHD, CTD, and TAA. Testing NOTCH1 for an early diagnosis in LS-CHD/RS-CHD/CTD/TAA is warranted.Genet Med 18 9, 914-923.

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Year:  2016        PMID: 26820064     DOI: 10.1038/gim.2015.193

Source DB:  PubMed          Journal:  Genet Med        ISSN: 1098-3600            Impact factor:   8.822


  42 in total

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8.  Identification of de novo mutations and rare variants in hypoplastic left heart syndrome.

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Review 8.  Bicuspid Aortic Valve: a Review with Recommendations for Genetic Counseling.

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9.  Functional genomics and gene-environment interaction highlight the complexity of congenital heart disease caused by Notch pathway variants.

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10.  Importance of complete phenotyping in prenatal whole exome sequencing.

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