Rashi Sharma1, Shweta Rehani2, Monica Mehendiratta3, Priyanka Kardam4, Madhumani Kumra5, Yulia Mathias6, Jyoti Yadav7, Khushboo Sahay8. 1. Senior Lecturer, Department Oral Pathology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, Haryana, India . 2. Reader, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research , Faridabad, Haryana, India . 3. Reader, Department of Oral Pathology, ITS Dental College , Greater Noida, Uttar Pradesh, India . 4. Senior Lecturer, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research , Faridabad, Haryana, India . 5. Professor, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, Haryana, India . 6. Professor and Head, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences & Research , Faridabad, Haryana, India . 7. Senior Lecturer, Department of Oral Pathology and Mirobiology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, India . 8. Senior Lecturer, Department of Oral Pathology, Sudha Rustagi College of Dental Sciences and Research , Faridabad, Haryana, India .
Abstract
INTRODUCTION: Collagen degradation is important both for carcinogenesis and in its progression. Research regarding the co-relation of collagen with Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) is less explored. AIM: To elucidate the nature of collagen in Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) using Picrosirius Red Stain (PSR) under polarizing microscopy. MATERIALS AND METHODS: The study consisted of a total 40 samples which were divided into three groups. Group I included buccal mucosa as negative and irritation fibroma as positive control, group II consisted of OED and group III consisted of Oral Squamous Cell Carcinoma (OSCC). A histochemical analysis was conducted using PSR-polarization method by two independent observers. RESULTS: The control group shows predominantly reddish-orange birefringence. In OED with the advancement of grades, the colour changed from yellowish-orange colour to yellow-greenish with progressive increase in greenish hue. As OSCC regresses from well to poorly differentiated, the colour changed from reddish-orange to yellowish orange to greenish-yellow suggesting a transition from mature to immature collagen. CONCLUSION: An observable gradual change in collagen of both OED and OSCC was noted as they were proceeding from benign to critical step. Thus, PSR is a useful tool for studying stromal changes as supporting collagen shows the transition in the form besides the alterations in epithelial cells.
INTRODUCTION: Collagen degradation is important both for carcinogenesis and in its progression. Research regarding the co-relation of collagen with Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) is less explored. AIM: To elucidate the nature of collagen in Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) using Picrosirius Red Stain (PSR) under polarizing microscopy. MATERIALS AND METHODS: The study consisted of a total 40 samples which were divided into three groups. Group I included buccal mucosa as negative and irritation fibroma as positive control, group II consisted of OED and group III consisted of Oral Squamous Cell Carcinoma (OSCC). A histochemical analysis was conducted using PSR-polarization method by two independent observers. RESULTS: The control group shows predominantly reddish-orange birefringence. In OED with the advancement of grades, the colour changed from yellowish-orange colour to yellow-greenish with progressive increase in greenish hue. As OSCC regresses from well to poorly differentiated, the colour changed from reddish-orange to yellowish orange to greenish-yellow suggesting a transition from mature to immature collagen. CONCLUSION: An observable gradual change in collagen of both OED and OSCC was noted as they were proceeding from benign to critical step. Thus, PSR is a useful tool for studying stromal changes as supporting collagen shows the transition in the form besides the alterations in epithelial cells.
Authors: Miguel Á Manzanares; Akihiro Usui; Deanna J Campbell; Catherine I Dumur; Gabrielle T Maldonado; Michel Fausther; Jonathan A Dranoff; Alphonse E Sirica Journal: Am J Pathol Date: 2017-03-15 Impact factor: 4.307
Authors: Marja B Pimentel; Fernando T P Borges; Fouad Teymour; Olga Y Zaborina; John C Alverdy; Kuili Fang; Seok Hoon Hong; Austeja Staneviciute; Yusheng J He; Georgia Papavasiliou Journal: J Mater Chem B Date: 2020-03-25 Impact factor: 6.331